摘要
目的:探讨细胞因子信号转导抑制子3(SOCS3)在实验性重症急性胰腺炎(SAP)中的表达和作用.方法:32只♂Sprague-Dawley大鼠随机分为对照组(NC组)和3组SAP6h、12h、18h组,每组8只.以4%牛磺胆酸钠胰胆管逆行注射诱导SAP模型,动态测定各组血清淀粉酶(AMY)水平;光镜下观察胰腺大体及组织学表现;ELISA方法测定大鼠血清中IL-6、IL-18的表达情况;蛋白印迹法(Western blotting)和免疫组织化学法测定胰腺组织中SOCS3的定位和表达.结果:与NC组相比,SAP各组AMY水平均明显升高(2675.18±278.32,3541.15±215.43,4568.89±357.86vs651.38±52.94,均P<0.05);光镜下胰腺组织损伤随病情进展而逐渐加重;各组血清中均有IL-6、IL-18表达;与NC组比较,SAP各组IL-6、IL-18表达水平显著上调(P<0.05);NC组有极少量的SOCS3表达,SAP各组SOCS3蛋白表达明显高于NC组,且随造膜时间延长逐渐增高(P<0.05);SOCS3表达变化与胰腺组织的严重程度和血清炎症因子的变化均一致.结论:SOCS3在SAP的发病和病情变化中起到非常重要的抑炎作用.
AIM：To investigate the mechanism of action of suppressor of cytokine signaling 3（SOCS3） in experimental severe acute pancreatitis（SAP） in rats.METHODS：A rat model of SAP was reproduced by retrograde injection of 4%sodium taurocholate into the biliopancreatic duct.Thirty-two male SD rats were randomly and equally assigned into four groups：normal control group（NC） and three SAP groups（6,12 and 18 h） .Serum amylase（AMY） was measured dynamically.Pathological changes in the pancreas and lung were observed under a light microscope.The concentrations of IL-6 and IL-18 were determined by ELISA.The localization and expression of SOCS3 protein in the pancreas were detected by immunohistochemical staining and Western blotting.RESULTS：Compared to the NC group,serum level of AMY increased significantly in the three SAP groups（2675.18±278.32,3541.15±215.43,4568.89±357.86 vs 651.38±52.94,all P0.05） . Pancreatic injuries revealed under a light microscope were gradually aggravated with disease progression.Serum concentrations of IL-6 and IL-18 in the SAP groups increased significantly compared to the NC group（all P0.05） .The levels of SOCS3 protein in the SAP groups increased significantly compared to the NC group（all P0.05） .The changes in SOCS3 protein expression were correlated with the severity of pancreatic injury and serum concentrations of IL-6 and IL-18.CONCLUSION：SOCS3 plays an important role in inhibiting inflammatory reaction in rats with SAP.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第31期3212-3216,共5页
World Chinese Journal of Digestology
基金
南京军区南京总医院科研基金资助项目
No.2009M012~~
