摘要
目的分析类风湿关节炎(RA)患者滑膜液及血清中干扰素(IFN)-γ诱导单核细胞因子(MIG)水平变化,探讨MIG在RA的临床意义。方法应用酶联免疫吸附法(ELISA)技术检测RA患者滑膜液及血清中MIG及IFN-γ水平,分析MIG与IFN-γ及临床指标的关系。结果滑膜液中,RA患者MIG及IFN-γ水平明显高于对照组(P均<0.01),MIG与IFN-γ水平呈正相关(r=0.486,P=0.035);血清中,RA患者活动组MIG及IFN-γ水平明显高于稳定组(P均<0.01),而后者又明显高于对照组(P均<0.01),RA活动期患者MIG与IFN-γ水平呈正相关(r=0.304,P=0.043);30例活动期RA患者治疗前MIG及IFN-γ水平明显高于治疗后(P均<0.01),且治疗前后MIG水平的变化与RF、ESR、CRP的变化无相关性(P均>0.05),与DAS28的变化呈正相关(r=0.405,P=0.029)。结论 RA患者的血清及滑膜液中存在异常增高的MIG,且其水平与病情活动相关,可作为RA病情活动新的参考指标,血清中MIG水平变化可为RA的疗效提供参考。
Objective To analysis the level of monokine induced by IFN-γ（MIG） in synovial fluid and serum from patients with rheumatoid arthritis（RA） and to investigate it′s clinical significance.Methods The levels of MIG and IFN-γ in serum and synovial fluid from RA patients were tested by technique of ELISA.Simultaneously the relationships among MIG,IFN-γ and other clinical indexes were analyzed.Results MIG and IFN-γ in synovial fluid from patients with RA were significantly higher than those in control groups（P0.01）,and there was a positive correlation between them（P=0.035）.The levels of MIG and IFN-γ in active RA patients′ serum,which were proved positively related（P=0.043）,were apparently higher than those in stable ones（P0.01）,meanwhile the same case occurred between stable and control groups（P0.01）.MIG and IFN-γ from 30 active RA patients were tested much higher than those after treatment（P0.01）,and it also showed MIG had no correlation with RF,ESR,CRP and positive relationship with DAS28（P=0.029）.Conclusion MIG,which has abnormal elevations in synovial fluid and serum,can be considered as the new conference index for RA,and its level in serum may play an important role in monitoring the therapeutic effect.
出处
《医学研究杂志》
2011年第12期87-89,共3页
Journal of Medical Research
