摘要
目的探讨异丙酚重复麻醉对新生大鼠海马钙离子，钙调蛋白依赖性激酶Ⅱα（CaMKⅡα）表达的影响。方法新生7d健康SD大鼠42只，体重12—16g，雌雄各半，采用随机数字表法，将其随机分为2组（n=21）：对照组（C组）和异丙酚重复麻醉组（P组）。C组腹腔注射生理盐水7．5ml／kg，连续7d；P组腹腔注射异丙酚75ml／kg，连续7d。出生后28d时采用Morris水迷宫实验检测认知功能。水迷宫实验结束24h时断头处死大鼠，取脑组织，分别采用免疫组织化学法和Western bolt法检测海马CAl区CaMKⅡα及磷酸化CaMKIⅡα（pCaMKⅡα）的表达。结果与C组比较，P组逃逸潜伏期延长，空间探索时间缩短，海马CAl区CaMKⅡα和pCaMKⅡα表达下调（P〈0．01）。结论异丙酚重复麻醉降低新生大鼠认知功能的机制可能与下调海马CaMKⅡα的表达和抑制其活性有关。
Objective To investigate the effect of propofol repeated anesthesia on the expression of CaMK Ⅱα in the hippocampus in neonatal rats. Methods Thirty-two SD rats aged 7 days weighing 12-16 g were randomly divided into 2 groups （n = 16 each）： group C received intraperitoneal 0.9% normal saline 7.5 ml/kg once a day for 7 days and group P received propofol 75 mg/kg once a day for 7 days. Learning and memory function were assessed using Morris warter maze at 28 days old of rats. The animals were sacrificed at 24 h after the tests and brain tissues were removed. The expression of CaMK Ⅱα and phosphorylated CaMK Ⅱα （pCaMK Ⅱα） in hippoeampal CA1 region were determined by immunochemistry and Western boh. Results Compared with group C, the escape latency was significantly prolonged, space exploration time shortened and expression of CaMK Ⅱα and pCaMK Ⅱα down-rugulated in group P than in group C （ P 〈 0.01 ）. Conclusion Propofol repeated anesthesia decreases congnitive function through down-regulating the expression and inhibiting the activity of CaMK Ⅱαin hippoeampus in neonatal rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第11期1335-1337,共3页
Chinese Journal of Anesthesiology
关键词
二异丙酚
婴儿
新生
钙-钙调素依赖性蛋白激酶2型
海马
Propofol
Infant, newborn
Calcium-ealmodulin-dependent protein kinase type 2
Hippocampus
