摘要
目的探讨针刺益气活血法对血管性痴呆(VD)大鼠学习记忆能力和脑海马组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的影响。方法将SD大鼠30只随机分为模型组、治疗组及对照组,每组各10只。模型组及治疗组采用改良的Pulsinelklli-4血管阻断法建立VD大鼠模型;治疗组造模后选用百会、膈俞、气海、三阴交、膻中组成益气活血方针刺治疗;未造模大鼠作为对照组。观察各组大鼠Morris水迷宫实验各项指标及脑海马组织SOD活性和MDA含量的变化。结果模型组大鼠每日逃避潜伏期均明显长于对照组(P<0.01),治疗组大鼠逃避潜伏期较模型组明显缩短(P<0.01),但仍长于对照组(P<0.01)。3组大鼠在训练第5 d后通过原平台位置的次数,治疗组大鼠多于模型组(P<0.01),但低于对照组(P<0.05)。模型组脑海马组织中的SOD活性较对照组明显降低,而MDA含量明显增高(P<0.01)。治疗组与模型组比较能明显升高SOD活性,降低MDA含量(P<0.01),并且治疗组升高的SOD活性、降低的MDA含量已接近正常组(P﹥0.05)。结论针刺益气活血法治疗血管性痴呆的作用可能与其改善VD大鼠学习记忆能力,显著提高脑海马组织中SOD活性及降低MDA含量有关。
Objective To investigate the effect of acupuncture therapy with Qi-supplementing and blood-invigorating on ability of learning and memory and superoxide dismutase and malondialdehyde in hippocampal tissue of rat with vascular dementia（VD）.Methods 30 SD rats were randomly divided into 3 groups of 10.VD model rats were established by Pulsinelklli-4 vessel occlusion in model group and treatment group.Treatment group received Yiqihuoxue prescription which was composed of Baihui,Geshu,air sea,Sanyinjiao and Shanzhong.Morris water maze test indicators,SOD activity and MDA content in hippocampus were detected in rats of three groups.Results Daily escape latency of rats in model group were significantly longer than that in control group（P0.01）,escape latency of rat in treatment group was significantly shorter than that in the model group（P0.01）,but still longer than that in the control group（P0.01）.The numbers of rat passing original platform in treatment group was more than that in model group after the first 5 days（P0.01）.SOD activity in model group was decreased and MDA was increased in comparison with those in control group（P0.01,P0.05）.SOD activity in model group was increased and MDA was decreased in comparison with those in model group（P0.01）.And increased SOD activity and reduced MDA levels in the treatment group were closed to those of normal group（P 0.05）.Conclusion The role of acupuncture therapy with Qi-supplementing and blood-invigorating on vascular dementia maybe related to improving ability of learning and memory of rat with VD,enhancing SOD activity and decreasing MDA content in hippocampal tissue of rat.
出处
《河北中医》
2011年第10期1554-1556,共3页
Hebei Journal of Traditional Chinese Medicine
基金
陕西省教育厅科研资金资助项目(编号:06JK314)
