摘要
目的:探讨相同浓度不同生物群解脲支原体血清型3和8(Uu3、Uu8)的致病性差异、两群混合感染时与单一感染的区别。方法:将52只经阴道脱污染及雌激素预处理过的母兔随机分为A,B,C和D组(对照组),阴道分别接种相同浓度(106拷贝/g)的Uu3,Uu8,Uu3和Uu8的混合液及无菌培养液。接种后第1,3,7,14,21,35,49,63,77,91和105天,取宫颈管分泌物行Uu的荧光定量聚合酶链反应(FQ-PCR)检测;于接种后第14天及实验结束时取各组8只及对照组2只兔的宫颈、子宫内膜及输卵管行HE染色光镜镜检,取第14天的兔宫颈管黏膜扫描电镜,观察急慢性炎症反应。结果:①A,B和C组母兔宫颈管分泌物中Uu总感染阳性率分别为27.3%(25/88),55.7%(49/88)和69.3%(61/88),差异有统计学意义(P<0.05)。其中接种后第1天阳性率达最高峰,A,B和C组的阳性率分别为87.5%(7/8),100.0%(8/8)和100.0%(8/8),此后随着接种后时间的延长,阳性率呈逐渐下降趋势。对照组在任何时间点均未检测出Uu。②A,B和C组母兔在接种后各时间点(第35天后除外),宫颈管UuFQ-PCR定量值差异无统计学意义(P>0.05),A,B和C组定量值均于接种后第1天达最高峰,分别为364×104拷贝/g,401×104拷贝/g,493×104拷贝/g,此后随接种后时间的延长定量值逐渐下降,同一实验组Uu在接种后的不同时间点浓度差异有统计学意义(P<0.05)。③A,B和C组的总致病率分别为31.3%(5/16),62.5%(10/16)和81.3%(13/16),差异有统计学意义(P=0.015),C组致病率最高,A组最低;3个实验组接种Uu后第14天的致病率差异无统计学意义(P>0.05),但在接种Uu后第105天时致病率差异有统计学意义(P=0.041)。D组实验全程结束均无致病。结论:①单一接种106拷贝/g的Uu3、Uu8及两群Uu混合接种对母兔均可表现出致病性。混合组致病性最强,Uu8组次之,Uu3组最弱。②在母兔生殖道中,接种相同浓度的Uu后,以混合组感染力及定植力最强,Uu8组次之,Uu3组最弱。提示U
Objective：To study the pathogenicity of Ureaplasma urealyticum serotype 3（Uu3） and 8（Uu8） with the same concentration in Female Rabbits,s Reproductive tract.Methods：Fifty-two oestradiol-treated adult female rabbits were randomly divided into group A,B,C and D.Ureaplasma urealyticum serotype 3,8,3 and 8,with the same concentration of 106copy/g while 4 rabbits of control group（group D） inoculated sterile medium.They were detect Uu from cervical secretions by fluorescent quantitative polymerase chain reaction（FQ-PCR） at 1,3,7,14,21,35,49,63,77,91,105 days.They were necropsied at 14,10^5 days of postinoculation randomly to observing tissues of cervix,endometria,fallopian tube by light microscope and cervical mucosa by scanning electron microscope.Results：①The total positive rabbits of Uu in three experimental groups show a significant difference（P〈0.05） ,which were 27.3%（25/88） ,55.7%（49/88） ,69.3%（61/88） .But before 35 days,the positive rate of the three experimental groups at the same time point in all of Female rabbits infected cervical secretions does not show a statistically significance（P〈0.05） ,and the positive rate descended as the days of postinoculation lasting.The highest positive rates of three experimental groups were 87.5%（7/8） ,100.0%（8/8） ,100.0%（8/8） at 1 d after inoculation,but the conlisation didn＇t persist at 105 d.All rabbits of control group were negative.②There was not statistical difference in the quantity of Uu among experimental groups（P〈0.05） .Their peak level were 364×10^4copy/g,401×10^4copy/g,493×10^4copy/g,reached at 1d,but the FQ-PCR values reduced as the days of postinoculation lasting.In the same experimental group at different time points,the concentrations was a significant difference（P〈0.05） .③The total pathogenicity rates of three groups were significantly different（P=0.015） ,which were 31.3%（5/16） ,62.5%（10/16） ,81.3%（13/16） .But there were not statistical difference among experimenta
出处
《国际妇产科学杂志》
CAS
2011年第6期520-523,540,共5页
Journal of International Obstetrics and Gynecology
基金
湖南省2011科技基本建设项目(20111318)
关键词
解脲支原体
血清分型
聚合酶链反应
毒力
模型
动物
Ureaplasma urealyticum
Serotyping
Polymerase chain reaction
Virulence
Models
animal
