摘要
以从西藏藏族自治区引种的无毛漆姑草带腋芽茎段为外植体进行组织培养试验,先后开展了初代培养筛选、继代增殖、生根和移栽等,以筛选出无毛漆姑草适宜的增殖、生根培养基以及移栽基质.结果表明:在丛生芽诱导阶段,细胞分裂素6-BA是主导因子,最适增殖培养基为MS+6-BA0.5mg/L+NAA0.1mg/L+30g/L蔗糖,增殖系数达5.12,丛生芽长势良好,适宜移栽;生根培养阶段,附加NAA的培养基生根率和根生长状况普遍较单独附加IBA的差,IBA质量浓度为0.5mg/L时根生长综合指数最高,最适生根培养基为MS+IBA0.5mg/L+20mg/L蔗糖;移栽阶段,根长≥2.5cm的组培苗在腐叶土∶蛭石=3∶1的混合基质中成活率最高,达100%.
The tissue culture of Sagina saginoides from Tibet was studied systematically from aspects of disposal,shoot inducement,proliferation and rooting to transplantation.The results showed that in the proliferation period,6-BA was a major factor,the suitable medium was MS＋6-BA0.5 mg/L＋NAA 0.1 mg/L＋30 g/L sugar,in which the propagation coefficient reached to 5.12,and the buds grew well,so the medium was suitable for transplantation.In the rooting period,the rooting rates of medium contained NAA and IBA were all lower than that of only contained IBA,the suitable medium was 1/2 MS＋IBA 0.5 mg/L＋20 mg/L sugar.In the transplantation period,the survival rate of plantlets with 2.5 mm of root length reached 100% in the mixture soil（leaf mold∶vermiculite as 3∶1）.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2011年第6期66-72,共7页
Journal of Gansu Agricultural University
基金
国家林业公益项目(200704043)
关键词
无毛漆姑草
组织培养
增殖
生根
移栽
Sagina saginoides
tissue culture
proliferation
rooting
transplantation
