摘要
目的建立同时测定苦碟子注射液中绿原酸、木犀草素-7-O-龙胆二糖苷、木犀草素-7-O-β-D-吡喃葡萄糖苷和木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷含量的HPLC法。方法色谱柱为Agela Pro-mosil C18柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-体积分数为1.0%乙酸水溶液(B),梯度洗脱;流速为1.0 mL.min-1;检测波长为350 nm。结果绿原酸、木犀草素-7-O-龙胆二糖苷、木犀草素-7-O-β-D-吡喃葡萄糖苷和木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷的线性关系良好,线性范围分别为0.730～5.84 mg.L-1、0.530～4.24 mg.L-1、0.990～7.92 mg.L-1和2.00～16.0 mg.L-1;平均回收率分别为98.8%、99.9%、99.6%和100.6%,RSD分别为0.8%、1.1%、1.0%和0.6%(n=5)。结论该法简便、可行,重现性好,结果准确,为苦碟子注射液质量控制提供参考依据。
Objective To develop a method for the quantitative analysis of chlorogenic acid, luteolin-7-O- gentibioside, luteolin-7-O-β-D-glucopyranoside ,luteolin-7-O-β-D-glucuronopyranoside in the injection of Ixeris sonchifolia simultaneously. Methods The injection was determined by Agela Promosil C18 column （250 mm ×4. 6 mm,5 μm） ;the mobile phase consisted of acetonitrile（A） and 1.0% HOAc（B） with gradient elution was used;the flow rate was 1.0 mL.min-t. The detector wavelength was set at 350 nm. Results The linear response ranges were in 0. 730- 5. 84 mg·L^-1,0. 530- 4. 24 mg ·L^-1,0. 990- 7.92 mg·L^-1, 2. 00-16. 0 mg·L^-1, respectively. The recovery ranges were 98.8 %, 99. 9 %, 99.6 %, 100. 6 %, respectively. The relative standard deviations （RSDs） were 0. 8 %, 1.1%, 1.0%, 0. 6 % （ n = 5 ）, respectively. Conclusions This method can be used for the quality control of the injection of of Ixeris sonchifolia（ Bge. ）Hance.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2012年第1期70-73,80,共5页
Journal of Shenyang Pharmaceutical University
