摘要
产氢菌株Clostridium sp. T7分离自天津海水浴场潮间带的污泥.研究起始pH、碳源、氮源、NaCl质量分数对菌株T7产氢性质的影响.结果表明:菌株T7最适产氢的起始pH是6.0,能够利用蔗糖、葡萄糖和果糖等碳源发酵产氢.菌株T7能够利用牛肉膏和酵母粉为单一氮源产氢,不能利用蛋白胨为氮源进行产氢.NaCl质量分数能影响菌株T7的产氢量,海水培养条件(NaCl质量分数为3%)下,最高产氢量是每摩尔葡萄糖(1.48,±0.05)mol,相比之下,淡水培养条件下其产氢量提高20%.NaCl质量分数在0.4%～7%时,菌株T7都能够产氢,这表明菌株T7有望应用于淡水或高盐有机废水产氢领域.
A hydrogen-producing bacterium,Clostridium sp.T7,was isolated form the sludge collected from intertidal zone of a bathing beach in Tianjin.The effects of initial pH value,carbon source,nitrogen source and NaCl concentration on hydrogen production of strain T7 were determined in this study.The results showed that strain T7 produced hydrogen production at maximal level when growing at initial pH of 6.0.The strain was able to produce hydrogen using many carbon sources,such as glucose,sucrose and fructose.The strain T7 could produce hydrogen by using yeast extract and beef as ni-trogen source,but not peptone.The level of hydrogen production was affected by the salt concentration.In marine condi-tions（NaCl concentration as 3%）,hydrogen production was（1.48±0.05）mol/mol glucose,whereasi,t increased by 20%in fresh conditions.It could produce hydrogen at the NaCl concentration from 0.4%to 7%.As a result,Clostridium sp.T7 can be considered to be used for bio-hydrogen production and biological treatment of fresh or marine organic waste.
出处
《天津科技大学学报》
CAS
2011年第6期12-15,共4页
Journal of Tianjin University of Science & Technology
基金
天津市海洋资源与化学重点实验室开放基金(200912)
天津科技大学实验室开放基金(1105A213)
关键词
潮间带污泥
产氢菌株
产氢量
intertidal sludge
hydrogen-producing strain
hydrogen production
