摘要
目的 :研究和探讨癌肿宁治疗实验性肝癌的疗效和机制。方法 :建立荷实体型肝癌 (H2 2 )小鼠模型 ,观察经癌肿宁治疗对其肿瘤红细胞免疫和肿瘤细胞周期的影响。结果 :癌肿宁可显著提高荷瘤机体肿瘤红细胞免疫功能 ,其高、低剂量组的 3个肿瘤红细胞花环率 (ETER、ATER、DTER)均显著高于模型组和阳性对照组 (P<0 .0 0 1) ,且有的已明显接近或超过正常组 ;经流式细胞术分析发现 ,癌肿宁高、低剂量组的 S期肿瘤细胞较模型组为高 ,而 G2 加 M期细胞明显少于模型组 ,表明癌肿宁可影响肿瘤细胞周期从 S期向 G2 加 M期的转变过程 ,将细胞阻滞在 S期 ,从而抑制肿瘤细胞的增殖。结论 :癌肿宁有明显的抗肝癌作用 。
Objective:TO observe the mechanism and effect of the composite prescription Aizhongning(AZN) on experimental hepatocarcinoma.Methods:The model of hepatocarcinoma 22 solid tumor bearing mice was produced and treated with AZN.The tumor erythroyte immune and cell cycle were observed.Results:AZN could significantly promote the immunoadhesion ability of red blood cells and attack tumor cells.And the ETER、ATER、DTER were significantly different between comparing model group and 5 FU group (P< 0.001 ).Furthermore,AZN could obstruct the transformation of tumor cell cycle from S phase to G 2+M phase, which significantly inhibited the proliferation of tumor cells.Conclusion:AZN had obvious anti hepatocarcinoma effect and could significantly promote the tumor erythroyte immune function of tumor bearing mice.
关键词
癌肿宁
肝肿瘤
细胞免疫
细胞周期
Aizhongning
hepatocarcinoma mice
tumor erythroyte immune
cell cycle
