摘要
引言
好氧甲烷氧化菌在新陈代谢上具有独一无二的特性：它们能够利用甲烷和其他一碳化合物作为唯一碳源和能源。这类微生物最典型的特点是利用甲烷单加氧酶（MMO,methane monooxygenase）催化甲烷氧化为甲醇。
Facultative methanotrophs can utilize methane as well as multi-carbon compounds,including organic acids and carbohydrates.Facultative methanotrophs from aged-refuse were enhanced in situ to overcome the limitations of methane oxidation by existing landfill covers.Methanotrophs from oligotrophic aged-refuse had a better environmental tolerance by analysis of scanning electron microscope（SEM）.These bacteria could enrich quickly in the presence of carbon source.However,the methane-oxidation capability could not be improved if only adding carbohydrates or nitrate mineral salts（NMS） medium alone.Compound acclimation of facultative methanotrophs were carried out by NMS medium and glucose/starch.After a delay period of 7—9 d,methane consumption came into a logarithmic growth period,which indicated that facultative methanotrophs had strong biological activity and high substrate competitive advantage.Metabolic pathways of facultative methanotrophs were modified by adding NMS medium,and methane could be utilized despite the presence of other carbon sources.The activity of facultative methanotrophs was enhanced by low concentration of chloroform.Oxidation rate of methane reached 0.114 ml·d-1·g-1 as the concentration of chloroform was 50 mg·L-1.The problems about the engineering application of obligate methanotrophs were overcome due to the discovery of the new method,and the results would have important implication for understanding the methane-oxidizing bacteria and the factors controlling methane fluxes in the environment.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2012年第1期266-271,共6页
CIESC Journal
基金
国家自然科学基金项目(51008322)
重庆市自然科学基金项目(2010BB7300&5309)
重庆教委基金(KJ100803&110812)~~
