摘要
目的:研究纳米银、载银纳米二氧化钛、掺氮纳米二氧化钛对粪肠球菌的抗菌作用。方法:将粪肠球菌在MBECTMP&G Assay中培养24 h,构建粪肠球菌感染模型,扫描电镜观察MBECTMP&G Assay上粪肠球菌感染情况。应用MBECTMP&G Assay以连续稀释法检测纳米银、载银纳米二氧化钛、掺氮纳米二氧化钛的最小抑菌浓度(MIC)和最小生物膜清除浓度(MBEC)。结果:扫描电镜下见粪肠球菌在MBECTM P&G Assay上形成生物膜。纳米银对粪肠球菌的MIC和MBEC分别为62.5 mg/L和1 000 mg/L,载银纳米二氧化钛MIC为64 g/L,但在所用浓度范围内均不能清除生物膜细菌,即未测得MBEC;掺氮纳米二氧化钛的MIC、MBEC均未测得;次氯酸钠的MIC和MBEC分别为6.56 g/L和13.13 g/L。结论:纳米银对粪肠球菌有较强抗菌作用,能清除粪肠球菌生物膜;粪肠球菌对载银纳米二氧化钛和掺氮纳米二氧化钛有较强抵抗力。
AIM： To evaluate the antibacterial effect of nano-silver,Ag-embedded nano-TiO2 and nitrogen-doped nano-TiO2 against E.faecalis.METHODS： E.faecalis was inoculated in MBECTM PG Assay to construct an E.faecalis infection model.E.faecalis infection on MBECTM PG Assay was observed by scaning electron microscope.The minimum inhibitory concentration（MIC） and minimum biofilm eradication concentration（MBEC） were detected by serial dilution method using MBECTM PG Assay.RESULTS： E.faecalis biofilm formed on MBECTM PG Assay.MIC and MBEC of nano-silver against E.faecalis were 62.5 mg/L and 1,000 mg/L respectively.MIC of Ag-embedded nano-TiO2 against E.faecalis was 64 g/L.MBEC of Ag-embedded nano-TiO2,MIC and MBEC of nitrogen-doped nano-TiO2 could not be detected in this study.MIC and MBEC of sodium hypochlorite were 6.56 g/L and 13.13 g/L.CONCLUSION： Nano-silver has strong antibacterial effect on E.faecalis and can eradicate E.faecalis biofilm.E.faecalis shows resistance against Ag-embedded nano-TiO2 and nitrogen-doped nano-TiO2.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2011年第12期694-697,共4页
Chinese Journal of Conservative Dentistry
基金
广东省科技计划项目(2010B031100023)
关键词
纳米银
二氧化钛
粪肠球菌
抗菌作用
nano-silver
titanium dioxide
Enterococcus faecalis
antibacterial effect
