摘要
[目的]探讨葡萄籽油(grape seedoil,GSO)对氯化镉(CdCl_2)诱导大鼠肝脏细胞DNA损伤的保护作用。[方法]将40只SPF级雌雄各半SD大鼠随机分为对照组(腹腔注射生理盐水和灌胃生理盐水)、染镉组(腹腔注射1.0mg/kg的CdCl_2和灌胃生理盐水)、GSO低、中、高剂量干预组(均腹腔注射1.0mg/kg CdCl_2,同时分别灌胃0.66、1.32、2.64mg/kgGSO),硫代巴比妥酸法检测肝中丙二醛(malondialdehyde,MDA)的含量,采用单细胞凝胶电泳技术(SCGE)检测各组肝脏细胞DNA损伤情况。[结果]1.0mg/kgCdCl_2可诱导大鼠肝脏细胞合成MDA,使其含量明显增加并加速DNA的氧化损伤;GSO高剂量干预组中MDA含量较染镉组差异具有统计学意义(P<0.05),3个GSO干预组MDA的清除率分别为54.55%、54.55%和100.00%,不同剂量的GSO对MDA清除呈较好的剂量-效应关系。各GSO干预组DNA氧化损伤较染镉组均明显下降(P<0.05)。[结论]一定剂量葡萄籽油可降低大鼠肝脏中MDA含量和镉致DNA氧化损伤,具有明显的抗脂质过氧化损伤的作用。
[ Objective] To explore the protective effect of grape seed oil （GSO） on DNA oxidative damage of liver cells in rats exposed to CdCl2. [ Methods ] A total of 40 SD rats of SPF grade were randomly divided into 5 groups, which were the control group （intraperitoneal injection and gastric perfusion of normal saline with same volume）, the CdCl2 exposure-positive group （intraperitoneal injection of 1.0mg/kg CdCl2 and gastric perfusion of normal saline with the same volume）, and the GSO low-, medium- and high-dose intervention groups （intraperitoneal injection of 1.0 mg/kg CdCl2 and gastric perfusion of 0.66 mg/kg, 1.32 mg/kg, 2.64 mg/kg GSO, respectively） with 8 of either gender in each group. Malondialdehyde （MDA） content in liver was measured by thiobarbituric acid （TBA） method. DNA oxidative damage was measured by single cell gel eletrophoresis （SCGE）. [ Results ] The 1.0 mg/kg CdCl2 induced a significant increase in MDA content and DNA oxidative damage. The difference of MDA contents between the GSO high-dose intervention group and the CdCl2 exposure-positive group was significant （P 〈 0.05）. The clearances in the GSO low-, medium- and high-dose intervention groups were 54.55%, 54.55% and 100.00%, respectively. The DNA damage significantly decreased in the GSO intervention groups in a dose-dependent manner, compared with the CdCl2 exposure-positive group （P〈0.05）. [ Conclusion ] A certain dose of grape seed oil can induce a decrease in MDA content and DNA damage in rat liver after cadmium exposure.
出处
《环境与职业医学》
CAS
北大核心
2011年第12期754-756,共3页
Journal of Environmental and Occupational Medicine
基金
广东省中医药局项目"MAPKs信号在葡萄籽原花青素影响DNA损伤中的作用研究"(编号:2010417)
