摘要
目的评价氧化酶法测定血清总胆红素(TBil)的性能。方法用氧化酶法测定血清TBil,探讨氧化酶法的精密度、线性、分析灵敏度、抗干扰性和回收率,并与改良J-G法进行比较。结果 TBil的高、低浓度样本批内CV分别为0.38%、0.78%,日间CV分别为1.78%、2.73%;平均回收率为101.2%;分析灵敏度为1.23μmol/L;线性范围为1.23～574.40μmol/L;2种方法相关性良好,直线回归方程为Y=1.105X-1.11,r=0.998。血红蛋白、维生素C和脂肪乳分别为4.8 g/L、500 mg/L和5%时对氧化酶法测定血清TBil不产生干扰。结论氧化酶法具有良好的精密度、线性范围和抗干扰能力,与改良J-G法具有很好的一致性,适用于临床常规检测。
Objective To evaluate the performance of serum total bilirubin（TBil） determination by oxidase method.Methods Serum TBil was determined by oxidase method,and the method′s precision,linearity,analytical sensitivity,anti-interference ability and recovery rate were evaluated.The method was compared with the modified J-G method.Results The within-run coefficients of variation（CV） of high and low TBil concentration samples were 0.38% and 0.78% respectively.The day to day CV of high and low TBil concentration samples were 1.78% and 2.73% respectively.The average recovery rate was 101.2%.The analytical sensitivity was 1.23 μmol/L,and the linear range was from 1.23 to 574.4 μmol/L.There was a good correlation between the 2 methods,and the linear regression equation was Y = 1.105X-1.11（r=0.998）.There was no interference when hemoglobin,vitamin C and fat emulsion were 4.8 g/L,500 mg/L and 5% for serum TBil oxidase.Conclusions Oxidase method has good precision,linear range and anti-interference ability and has a good consistency with the modified J-G method.It is suitable for routine clinical testing.
出处
《检验医学》
CAS
北大核心
2011年第12期811-813,共3页
Laboratory Medicine
关键词
总胆红素
氧化酶法
分析灵敏度
Total bilirubin
Oxidase method
Analytical sensitivity
