摘要
利用同步辐射红外显微光谱研究6-hydroxydopamine(6-OHDA)毁损内侧前脑束所致帕金森病大鼠脑海马区神经元的生物化学成分改变。研究样本为6-OHDA诱导的帕金森大鼠模型海马区神经元。与正常大鼠海马区神经元比较,PD大鼠样品在属于脂类的CH2反对称和对称伸缩振动的2 924和2 850cm-1的振动吸收积分面积以及在峰值位于1 736cm-1的伸缩振动吸收积分强度比正常大鼠都有增加,提示PD样品脂质含量升高,而在属于核酸的PO2反对称伸缩振动和对称伸缩振动的强度比正常大鼠样品下降,提示PD样品中核酸的含量比正常样品减少。蛋白质的振动没有发现异常。该研究表明帕金森病大鼠模型海马神经元存在生物化学成分改变,这种改变可能与神经元的病理改变密切相关。
Synchrotron radiation based-Fourier transform i nfrared microspectroscopy（SR-FTIR） was used to preliminarily investigate the b iochemical composition of the hippocampal neurons for 6-hydroxydopamine-lesion ed rats and normal rats.Spectral analysis showed that in PD samples,the CH2 asymmetric and symmetric vibrational absorption of integral area at 2 924 and 2 850 cm-1 and the intensity of C-O vibrational abso rption at 1 736 cm-1（assigned to the lipid functional group） increase com pared to normal samples,which indicate that lipid content increased in PD sampl e;the PO2 asymmetric and symmetric vibrational absorption decrease compared t o normal samples（assigned to the nucleic acid functional group;However no clea r difference of the vibrational fingerprinting of protein between PD and normal samples was noticed.The present results suggest that the changes in biochemical composition in hippocampal neurons in PD rats probed by synchrotron radiation b ased-FTIR may contribute to the elucidation of PD pathology.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2012年第1期113-117,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(30900422
11179019)
科技部(973)项目(2010CB529806)
上海市自然科学基金项目(11ZR1440500)
上海市教委分子生理学重点学科资助
关键词
帕金森病
海马神经元
生物化学成分
同步辐射红外显微光谱
Parkinson＇s disease
The hippocampal ne uron
Biochemical composition
Fourier transform infrared microspectroscopy
Synchrotron radiation
