摘要
本文报道了不同栽培番茄与秘鲁番茄(Lycopersicum peruvianum)PI128657中8号株系进行种间杂交,以授粉后25～30天的未成熟种子进行离体培养,均成功地获得大量的杂种株。诱导培养基为MS培养基附加2,4-D 2mg/l+2ip 1mg/l+椰乳100ml/l;分化培养基为MS培养基附加2mg/l玉米素;生根培养基为MS培养基附加mg/lIBA。并对F_1代再生株形态特性、细胞学、同功酶和抗性等进行观察和分析。
The interspecific cross between Lycopersico esculentum ( several cultivars) and L. peruvianvm ( strain 8 of variety PI 128657 ) was reported in this paper. The immature hybrid seeds of 25~30 days after pollination were cultured in vitro. The hybrid plants were all successful obtained.
Using MS medium as the basis medium, 2,4-D 2ip and cocoanut milk were applied for callus formation and bud differentiation. Adventitious buds were multiplied on MS medium containing ZT. MS medium with IBA was used as the rooting medium.
Morphological, cytological,resistant observation and electrophoretic analysis of isozyme peroxidase were made in F_1 regenerated plants.
出处
《园艺学报》
CAS
CSCD
北大核心
1990年第4期281-287,共7页
Acta Horticulturae Sinica
关键词
番茄
种间杂种
离体培养
再生株
Tomato
Interspecific hybrid
Tissue culture
Regenerated plant
