摘要
目的观察碱性成纤维细胞生长因子（bFGF）、血管内皮细胞生长因子（VEGF）、表皮细胞生长因子（EGF）、胰岛素及自体真皮颗粒对培养及移植的脂肪颗粒活性的影响因素。方法通过对培养中的幼鼠脂肪细胞，分别添加bFGF、VEGF、EGF、胰岛素及自体真皮颗粒，四甲基偶氮唑盐（MTT）法测定各组脂肪细胞活性，分析其作用效果；通过对移植体的大体观察、质量测定、HE染色、微血管数量及免疫组织化学染色等，比较各组添加物对脂肪组织活性的影响。结果除真皮颗粒组及对照组外，各实验组在不同时间点的最佳脂肪细胞活性浓度基本一致，且其细胞活性均高于对照组（P〈0．05）。动物实验中对照组可见较大区域坏死灶、囊样脂肪池。实验组脂肪细胞大小不一，存在大量的小体积脂肪细胞；除胰岛素组外，各实验组移植物残余质量及各组微血管密度均明显高于对照组，但随移植时间的延长差异逐渐缩小。结论bFGF、VEGF、EGF及胰岛素均能不同程度提高脂肪细胞活性，bFGF、VEGF、EGF及自体真皮颗粒均能不同程度地提高脂肪颗粒移植后的移植物质量，改善脂肪组织的血运重建。
Objective To observe the influence of basic fibroblast growth factor （bFGF）, vascular endothelial growth factor （VEGF）, epidermal growth factor （EGF）, insulin and self-corium granule on the fat granule under cultivating and after transplantation. Methods The adipocytes of mouse were treated with bFGF, VEGF, EGF, insulin and self-corium granule in different concentration gradient post inoculation, respectively, to determine their different cytoactivity with MTT method, and then draw their activity curve with time. The various grafts were implanted into 32 SD rats, which contained bFGF, VEGF, EGF, insulin, self-corium granule or physiologic saline, and then the general condition of animals, quality determination, pathological changes and immunohistochemical staining were observed. At last, the effects of the different implants on the fat granule were compared in different methods. Results The best cytoactive saturation of every experiental group was the same at different time points, except self-corium granule group, the cytoactivity of which was higher than that of control group （P〈0.05）, In animal experiment, focal necrosis and fat cistern were seen in comparatively larger area in the control group. The adipocyte of the experiental groups differed in size, and considerable small adipocytes were present. The capillary vessel density of all the experiental groups except the insuline group were higher than those of the control group （P〈0.05）. But the difference between the experiental groups and the control group became illegible gradually with time. Conclusions The activity of adipocyte being cultivated is differently increased by bFGF, VEGF, EGF and Insulin. The quantity of fat graft could be significantly raised by bFGF, VEGF, EGF and self-corium granule, which improve the revascularization of the fat graft after transplantation.
出处
《中华医学美学美容杂志》
2011年第6期437-440,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
