摘要
通过大孔树脂富集马兰粗提液中黄酮及三萜类化合物并对其进行体外抗脂质过氧化测定。比较了15种树脂的静态吸附和解吸附,以及吸附动力学、吸附等温线等性能,从中筛选出兼具富集黄酮类和三萜类化合物的最佳树脂AB-8。进而选择AB-8柱动态富集马兰中活性组分并对其进行活性组分的抗脂质过氧化测定。通过不同体积分数乙醇溶液洗脱显示:20%洗脱组分中黄酮类化合物含量最高,达到62.7%,同时含有19%的三萜类化合物,其总得率达到51.5%,60%洗脱组分中三萜类化合物含量最高,达到58.5%,总得率达到22.3%。同时,对所得主要组分进行抑制过氧化脂质活性实验,结果表明:两主要组分对体外组织(心、肝、肾、脑)的脂质过氧化均有一定的抑制效果,其中对肝脏的抑制效果最为明显。
Macroporous resin adsorption was used to purify flavonoids and triterpenoids from crude extract resulting from sequential organic solvent extraction of the whole plant of Kalimeris indica（L.） Sch-Bip.with 80% ethanol,petroleum ether（decolorization） and ethyl acetate and their lipid peroxidation inhibitory activity in vitro was measured.AB-8 was selected as the best resin out of 15 ones by comparing their static adsorption and desorption,adsorption kinetic characteristics and isothermal adsorption curves.Kinetic adsorption and desorption on AB-8 column were used to purify flavonoids and triterpenoids.The results of elution with different concentrations of ethanol showed that 20% ethanol eluate contained the largest amount of flavonoids,reaching 62.7%,and 19% triterpenoids with a total yield of 51.5% and that 60% ethanol eluate had the highest triterpenoid content,reaching 58.5% with a total yield of 22.3%.Both constituents had some inhibitory effect on lipid peroxidation in mouse heart,liver,kidney and brain tissues in vitro,especially on the liver.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第22期89-94,共6页
Food Science
