摘要
本研究从某农药厂污水处理池的活性污泥中分离得到1株能以乙羧氟草醚为唯一碳源生长的菌株。经生理生化鉴定和16S rRNA基因序列同源性分析,将此菌株初步鉴定为腐生葡萄球菌(Staphylococcus saprophyticus),并命名为YSC-1。对菌株YSC-1的生长特性研究表明:菌株的最佳生长温度和pH分别为30℃、7.0;NaCl浓度对菌株YSC-1生长有较大的影响。菌株在20℃～40℃之间均能降解乙羧氟草醚,在30℃、pH7.0的条件下对乙羧氟草醚的降解率最高;增加乙羧氟草醚的浓度会对菌株产生毒害作用,降低其降解率;提高接种量可以加快乙羧氟草醚的降解。在乙羧氟草醚终浓度为100 mg/L的工业废水经7d处理后,乙羧氟草醚的去除率达91.62%,说明菌株YSC-1在废水处理中具有很好的应用前景。
In this study, a bacterial strain using fluoroglycofen as sole carbon source was isolated from activated sludge of wastewater treatment tank in an insecticide factory. It was identified by physiological-biochemical identification and 16S rRNA gene sequences homology analysis, it belonged to Staphylococcus saprophyticus. The strain was named as YSC-1 and its growth was studied. The results showed that the optimum growth conditions for YSC-1 were 30℃ and pI-I 7. 0. The concentration of Nacl affected the growth significantly. The strain degraded fluoroglycofen at temperature of 20℃ -40℃ with the maximum fluoroglyoofen degrading rate at 30℃ and 7. 0, respectively. Increasing concentration of tluoroglycofen resulted in toxic effect on strain YSC-lto reduced the biodegradation rate. Increasing inoculums volume would speed up the degradation of tluoroglycofen. For industrial wastewater treated for seven days with final fluoroglycofen concentration of 100 mg/L, the removal rate reached 91.62%, which showed that the strain YSC-1 had a good application prospect for treatment of wastcwater.
出处
《四川环境》
2011年第6期7-12,共6页
Sichuan Environment
关键词
乙羧氟草醚
分离鉴定
生长特性
生物降解
Fluoroglycofen
isolation and identification
growth characteristics
biodegradation
