摘要
本研究旨在探索2-甲氧基雌二醇(2-ME2)联合硼替佐米对人骨髓瘤细胞株U266的协同抑制增殖和诱导凋亡作用及其可能机制。2-ME2、硼替佐米单用以及二者联合分别处理U266细胞,用CCK8方法检测细胞增殖活力,caspase3/7活性法检测细胞凋亡,流式细胞术分析细胞周期的变化,荧光实时定量PCR检测P21、BAX、BCL-2等mRNA水平变化。结果表明:相比于2-ME2和硼替佐米单用,2-ME2联合硼替佐米处理U266细胞后,细胞增殖明显抑制(p<0.05),细胞凋亡增加(p<0.05),细胞周期阻滞于G1-S期;在mRNA水平,P21及BAX表达上调,BCL-2表达下调。结论:2-ME2联合硼替佐米能更有效地抑制U266细胞增殖和诱导凋亡,具有协同作用,其机制可能与其诱导P21及BAX表达上调有关。
This study was aimed to explore the synergistic effect of 2-methoxyestradiol（2-ME2） and bortezomib（Bor） on the proliferative inhibition and apoptosis of U266 cell line and its possible mechanism.The cells were treated with 2-ME2,Bor alone and 2-ME2 combined with Bor,respectively.The cell viability and proliferative curve were detected by CCK8,the cell apoptosis was detected by caspase 3/7 activity test,cell cycle status was analyzed by flow cytometry,and real-time PCR was used to detect the mRNA expression of P21,BAX and BCL-2.The results showed that compared with cells treated with 2-ME2 or Bor alone,the proliferative potential of cells in combination group was significantly inhibited（p〈0.05）,and apoptosis rate markedly increased（p〈0.05）,cell cycle was arrested at G1-S phase,the mRNA expressive level of P21 and BAX increased,while the expression of BCL-2 decreased.It is concluded that 2-ME2 combined with Bor synergistically inhibits cell proliferation and induces apoptosis in U266 cell line.The possible mechanism may be associated with its effect of up-regulating P21 and BAX expressions.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第6期1424-1428,共5页
Journal of Experimental Hematology
基金
福建省自然科学基金项目资助
编号2009J01143
