摘要
本研究检测白血病患者骨髓中CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的表达水平,探讨白血病发生、发展、浸润、转移中CYR61、CTGF、VEGF-C、VEGFR-2蛋白的相互作用及其临床意义,寻找新的肿瘤标记物,为白血病的诊治提供新的思路。白血病74例,男38例,女36例,年龄6-77岁,中位年龄45岁。对照组12例,男7例,女5例。年龄16-78岁,中位年龄46岁。采用半定量逆转录聚合酶链反应(RT-PCR)方法测定CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的水平。结果表明,各组初发急性和慢性白血病患者骨髓中CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的表达与对照组相比均显著升高(p<0.05)。急性白血病缓解后CYR61和CTGF mRNA的表达高于对照组(p=0.039,0.025)。CTGF mRNA的表达在B-ALL组表达最高,高于AML、CML、CLL、T-ALL组(p=0.002,0.034,0.002,0.010)。AML组CYR61与CTGF、CYR61与VEGF-C、CTGF与VEGFR-2 mRNA的表达均呈正相关(r=0.452,0.466,0.464;p=0.045,0.038,0.039),CML组CYR61与VEGF-C mRNA的表达呈正相关(r=0.882,p=0.000)。急性白血病伴有髓外浸润者VEGF-C和VEGFR-2 mRNA的表达高于不伴有髓外浸润者(p=0.028,0.047)。AML组VEGF-C mRNA的表达与原始细胞数呈正相关(r=0.418,p=0.034)。结论:CYR61、CTGF及VEGF-C、VEGFR-2在白血病发病中相互作用,促进白血病发展、转移及浸润;且这些因子在不同类型白血病及髓外浸润中的作用存在差异。它们可能成为检测白血病的肿瘤标记物;阻断上述因子有可能阻断肿瘤的生长和转移。
The study was aimed to detect the levels of CYR61,CTGF,VEGF-C,VEGFR-2 mRNA in bone marrow（BM） of leukemia patients and investigate the interaction of CYR61,CTGF,VEGF-C,VEGFR-2 protiens in occurrence,development,infiltration and metastasis of leukemia and its clinical significance,to find a new tumor marker for diagnosis and treatment of leukemia with some new directions.74 patients with leukemia were enrolled in this study,38 out of them were males and 36 were females,aged from 6 to 77 years old with the median age of 45 years old.In the control group,7 males and 5 females,aged from 16 to 78 years old with the median age of 46.Semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） was used to detect the levels of CYR61,CTGF,VEGF-C,VEGFR-2 mRNA.The results showed that the levels of CYR61,CTGF,VEGF-C,VEGFR-2 mRNA in BM of newly diagnosed patients with acute and chronic leukemia of each group were significantly higher as compared with the control group（p〈0.05）.The levels of CYR61,CTGF mRNA in acute leukemia remission group were significantly higher than those in control group（p= 0.039,0.025）.The level of CTGF mRNA was highest in B-ALL group,and was higher than that in AML,CML,CLL,T-ALL groups（p=0.002,0.034,0.002,0.010）.In AML group,mRNA expressions of CYR61 and CTGF,CYR61 and VEGF-C,CTGF and VEGFR-2 were positively correlated（r=0.452,0.466,0.464;p=0.045,0.038,0.039）,and in CML group mRNA expression of CYR61 and VEGF-C was positively correlated（r= 0.882,p=0.000）. The expression levels of VEGF-C,VEGFR-2 mRNA in acute leukemia patients with extramedullary infiltration were higher than those in acute leukemia patients without extramedullary infiltration（p=0.028,0.047）.VEGF-C mRNA expression and the original cell counts in AML group were positively correlated（r= 0.418,p=0.034）.It is concluded that CYR61,CTGF,VEGF-C and VEGFR-2 interact each other in the pathogenesis of leukemia,promote the development,metastasis and infiltration of leukemia;and these factors in dif
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第6期1368-1373,共6页
Journal of Experimental Hematology
基金
内蒙古人才基金项目
