摘要
目的:应用microRNAs芯片筛选肾透明细胞癌细胞差异表达microRNAs,并寻找差异表达microRNAs调控的靶基因,为进一步研究其在肾透明细胞癌发病机制中的作用打下基础。方法:应用哺乳动物microRNAs表达谱芯片检测11位肾透明细胞癌患者癌组织及非肿瘤肾组织中microRNAs差异表达。结果:81种microRNAs在肾透明细胞癌组中有效表达,其中48种microRNAs仅在肾透明细胞癌组中有效表达;42种microRNAs在对照组中有效表达,9种microRNAs仅在对照组中有效表达。33种microRNAs在两组中均表达,其中19种microRNAs表达分析有显著差异。芯片显示结果经northern blot分析确认。结论:microRNAs在肾透明细胞癌细胞中异常表达,提示它们可能在肾透明细胞癌的发生发展中起着重要的调控作用。
Objective： To investigate the differentially expressed microRNAs and their target genes in renal clear cell carcinoma （RCCC）, which are useful for further studies on functions of microRNAs in pathogenesis of re nal clear cell carcinoma （RCCC）. Methods： The microRNAs expression profiles were analyzed in 11 pairs of RCCC and adjacent nontumorous tissue （NT）, from 11 RCCC patients, using a mammalian microRNAs microarray containing whole human mature and precursor microRNAs sequences. Results： 81 microRNAs were identified valid expression in RCCC samples,48 of which only detected in RCCC samples; 42 microRNAs identified valid expression in only NT samples,9 of which were found only in NT samples. In the 33 microRNAs found both in RCCC and NT samples expression levels of 19 microRNAs were analyzed significant difference. The chip results were confirmed by northern blot analysis. Conclusions：The expression profile of microRNA is changed in RCCC samples, which implies that those changed microRNAs may play an important role in the pathogenesis of RCCC.
出处
《临床泌尿外科杂志》
北大核心
2011年第12期940-943,957,共5页
Journal of Clinical Urology
关键词
肾肿瘤
透明细胞癌
微小RNA
差异表达
renal neoplasms
clear cell careinoma
microRNA
differential expression
