摘要
目的:探讨益肾降浊冲剂对阿霉素肾病大鼠肾小球硬化的干预作用与机制。方法:SD大鼠随机分为对照组、模型组、他汀组、益肾降浊冲剂(低、中、高剂量)组,每组6只。采用单侧肾切除加阿霉素重复注射建立肾小球硬化模型。用药8周后观察各组大鼠24h尿蛋白定量、血生化、氧化低密度脂蛋白(ox-LDL)的变化,PAS、Masson染色评估肾小球硬化程度,免疫组化观察肾小球氧化低密度脂蛋白受体(LOX-1)的表达,Western Blot检测Ⅰ型胶原(ColⅠ)、纤连蛋白(FN)的表达。结果:与对照组比较,模型组大鼠24h尿蛋白量、尿素氮(BUN)、肌酐(Scr)、胆固醇(Cho)、低密度脂蛋白胆固醇(LDL-C)、ox-LDL、肾小球硬化评分、系膜肾小球系膜基质比(M/G)均明显升高,肾小球LOX-1、ColⅠ、FN表达显著增加(P<0.01)。与模型组比较,益肾降浊冲剂组呈剂量依赖性降低24h尿蛋白量、Bun、Scr、Cho、LDL-C、ox-LDL水平以及肾小球硬化评分、M/G(P<0.01),下调LOX-1、ColⅠ、FN的表达(P<0.01)。结论:益肾降浊冲剂组能通过纠正脂代谢紊乱、抑制LOX-1的表达、减轻细胞外基质的沉积、改善肾小球硬化。
Objective： To investigate the protective effect and its possible mechanism of Yishenjiangzhuo Granules on renal glomerulosclerosis in the adriamycin（ADR）-induced nephropathy rats.Methods： The Sprague-Dawley（SD） rats were randomly divided into six groups： control group,model group,atorvastatin group,high,medium and low doses of Yishenjiangzhuo Granules group.Every group had six rats.The model was established by adriamycin injection and unilateral nephropathy.After treated for 8 weeks,PAS and Masson staining was used to assess glomerulosclerosis.The 24-hour urinary protein excretion and renal function were examined.Immunohistochemistry and Western Blot were used to messure the expression of LOX-1,ColⅠand FN.Results： Compared with control group,the 24-hour urinary protein excretion,BUN,Scr,Cho,LDL-C,ox-LDL was significantly increased in model group,as well as more severe glomerulosclerosis（P0.01）.The expression of LOX-1,ColⅠand FN were significantly enhanced（P0.01）.Yishenjiangzhuo Granules groups significantly decreased the above indexes in a dose dependent manner（P0.01）.Conclusion： Yishenjiangzhuo Granules can alleviate the dyslipidemia,down-regulate the expression of LOX-1,ColⅠand FN,and then inhibit glomerulosclerosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第12期2844-2848,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
福建省教育厅高校杰出青年人才计划项目(No.JA10125)
福建省卫生厅中医药科研课题重点项目(No.WZZSB0907)~~
