摘要
碳纤电极记录可用于对单个神经及内分泌细胞分泌过程进行电化学检测,其中技术关键之一是碳纤电极的制作。本文所介绍的碳纤电极的制作方法,简化了以往的工艺过程,使成功率与一致性得到了提高,同时检测灵敏度与噪声等指标满足了实验要求。用这样制作的碳纤电极在大鼠肾上腺嗜铬细胞上研究1甲基4苯基吡啶(MPP+)对于单胺能神经细胞递质胞吐过程的影响,发现MPP+对于胞吐没有直接的影响并且不增加高K+刺激所诱发的胞吐量。从而提示以往所报道的将MPP+通过灌流方法作用于多巴胺能神经核团,短时间内使组织液中多巴胺含量上升。
Carbon fiber electrode (CFE) can be used in detection of exocytosis of single neuron or endocrine cell. A simplified method for preparation of CFE, as described in the present paper, greatly ensures the coordination and success of the preparation. Such CFEs have a low noise level. Exocytosis of rat adrenal chromaffin cells was investigated in the clarification of the effect of MPP + on transmitter release. It is found that exocytosis was not stimulated by MPP + and the high K + induced secretion was not changed by MPP +. These observations suggest that the previously reported elevation of dopamine content in the surrounding histosolution of DA neuron is probably resulted from blockage of the re uptake of DA by MPP +.
出处
《生理学报》
CAS
CSCD
北大核心
1999年第6期692-699,共8页
Acta Physiologica Sinica
基金
国家自然科学基金!资助项目 (No 6 95710 14 3952 50 0 9
39480 0 0 8)
九五科技攻关计划! (96 90 6 0 5 0 8)
关键词
碳纤电极
分泌
肾上腺嗜铬细胞
电化学测量
MPP
carbon fiber electrode
neurotransmitter release
adrenal chromaffin cell
electrochemical detection
MPP +
