摘要
以高寒草地优势植物珠芽蓼(Polygonum viviparum)的一内生细菌菌株Z17为研究对象,采用平板对峙法测定其对7种植物病原真菌的抑菌能力,利用形态学和分子生物学2种方法确定其分类地位。该菌对玉米小斑病菌(Bipolaria maydis)、立枯丝核病菌(Rhizoctonia solani)、菌核病菌(Sclerotinia sclerotiorum)、西瓜尖镰孢(Fusarium oxysporumf.Niveum)、番茄早疫病菌(Alternaria solani)、番茄灰霉病菌(Botrytis cinerea)和小麦离蠕孢菌(Bipolaris sorokiniana)等均有抑制作用,抑菌谱较广。菌体杆状,菌体大小为(1.4～3.6)μm×(0.4～0.6)μm,革兰氏阳性,中生芽孢,与芽孢杆菌的形态一致;16SrDNA基因序列与莫海威芽孢杆菌(Bacillus mo-javensis)的模式菌株BCRC 17531相似度达99.79%,在系统发育树上Z17与菌株BCRC 17531的遗传距离小于0.000 5,故鉴定为莫海威芽孢杆菌。该菌可能具有开发生物农药的潜力。
Polygonum viviparum is the dominant species of alpine meadow community.A experiment was conducted to estimate the capacity of P.viviparum endophytic bacteria Z17 to antagonistic towards pathogenic fungi by PDA plates and to determine the taxonomic status of Z17 by morphological and molecular biology methods.The results of this study showed that Z17 inhibited Bipolaria maydis,Rhizoctonia solani,Sclerotinia sclerotiorum,Fusarium oxysporum f.Niveum,Alternaria solani,Botrytis cinerea,Bipolaris sorokiniana,indicating that Z17 had a wide antibacterial spectrum.Z17 was rod shape with a size of（1.4-3.6） μm ×（0.4-0.6） μm,Gram-positive,and produced spore in the center,and it was same morphological characteristics with Bacillus sp.Similarity between 16S rDNA gene sequence of Z17 and Bacillus mojavensis model strain BCRC 17531 reached 99.79%,and the genetic distance between Z17 and BCRC 17531 in the phylogenetic tree was less than 0.000 5.These results suggested that Z17 could be identified as Bacillus mojavensis and had the potential to be explored as Biopesticides.
出处
《草业科学》
CAS
CSCD
北大核心
2011年第12期2096-2101,共6页
Pratacultural Science
基金
国家自然基金项目(30471232)
甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目暨草业生态系统教育部省部共建重点实验室(CY-GG-2006-01)
关键词
高寒草地
内生拮抗菌
莫海威芽孢杆菌
鉴定
Alpine grassland
Antagonistic endophytic bacteria
Bacillus mojavensis
identification
