摘要
目的应用高效液相色谱法测定8种不同品种大枣中的芦丁含量并进行比较,为提取材料的选择提供依据。方法采用HPLC法,流动相为乙腈-0.4%醋酸水溶液(20∶80);柱温为室温;流速为1.0mL/min;检测波长为360 nm;进样量10μL。用外标法定量。结果各品种大枣芦丁的含量大小顺序依次为:陕北圆红,河北唐县(投料),新疆小枣,陕西马牙枣,河北沧州金丝小枣,新疆长红,陕西滩枣,新疆圆红。结论陕北圆红,新疆小枣和河北唐县的投料枣所含的芦丁较高。其中,陕北圆红的含量最高,为288.21μg/g。
Objective To compare the rutin contents in 8 kinds of Ziziphus jujuba by HPLC. Methods HPLC method was used with a mobile phase of acetonitrile -0.4% acetic acid （20：80）, column temperature （room temperature）, flow rate （1.0 mL/min）,detection wavelength （360 nm）,and injection volume （10μL）.Rutin was quantified by external standard method. Results The order of rutin content in 8 kinds was as follows： Red circle〉 Northern Hebei Tang County jujuba （feed）〉Xinjiang jujuba〉 jujuba Maya Shaanxi 〉Hebei Cangzhou Jinsixiaozao〉long red Xinjiang〉Shaanxi Beach date〉Xinjiang round red .Conclusion Content of rutin in Red circle, Xinjiang jujuba and Hebei Tang County jujuba were higher than the others. Among them, the content in Red circle was the highest with 288.21μg / g.
出处
《现代中药研究与实践》
CAS
2011年第6期69-71,共3页
Research and Practice on Chinese Medicines
