摘要
目的:探讨肿瘤相关抗原CA-X体内外表达的差异性。方法:采用蛋白质印迹法和流式细胞术分别检测肺腺癌细胞株移植裸鼠前后抗原表达的变化。结果:CA-X为高分子量糖蛋白,体外培养的SpcA1细胞株不表达CA-X,而一旦移植入裸鼠后,肿瘤重又表达该抗原。结论:肿瘤相关抗原CA-X移植入裸鼠后重新表达,说明CA-X的表达调控受体内某些因素的调控,也可能与细胞间信号转导有关。
Objective: To explore the differential expression of tumor associated antigen CA X in tumor cells in vitro and in vivo . Methods: Tumor xenografts of the human lung adenocarcinoma cell line SpcA1 were grown in athymia mice. The antigen was measured before and after xenograft by Western blotting and flow cytometric analysis. Results: The antigen CA X was a glycoprotein with a molecular weight of 170 000 to 200 000 . Human lung adenocarcinoma cell lines regain expression of antigen CA X when xenografted into athymia mice. Conclusion: These data indicate that CA X synthesis in human lung adenocarcinoma cells SpcA1 is regulated by factors presented only in the xenograft.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第12期983-985,共3页
Academic Journal of Second Military Medical University
