摘要
目的:探讨神经系统富亮氨酸重复超家族成员LRRN3膜蛋白在人胚脊神经节的表达和分布。方法:从人胚脊神经节分离mRNA和蛋白质,用RT-PCR、Western印记杂交法和免疫组织化学方法检测LRRN3膜蛋白表达。结果:LRRN3膜蛋白C端序列RT-PCR扩增产物cDNA在各胎龄脊神经节均有表达,长度约500bp;Western印记杂交结果显示LRRN3膜蛋白存在,分子质量约78 kD;免疫组织化学和免疫荧光组织化学结果表明LRRN3阳性表达细胞均为脊神经节感觉神经细胞,部分神经细胞弱阳性表达,部分未表达。结论:LRRN3膜蛋白在脊神经节神经元的表达,推断与脊神经节感觉神经元的发育、形态构建及损伤后修复有密切的关系。
Objective： To explore the expression and localization ofmembrance protein LRRN3 of dorsal root ganglion （DRG） in human embryo at the mRNA and protein levels. Methods： mRNA and protein of membrance protein LRRN3 were separated from DRG, and RT-PCR, Western Blotting, immunocytochemistry staining and Immunofluorescence were used to detect expression of membrance protein LRRN3. Results： RT-PCR production eDNA of LRRN3 C-terminal domain protein were detected in DRG at each gestational age, and the length is about 500 bp; Western blot analysis showed membrance protein LRRN3 were expressed and the relative molecular mass is about 78 kD; immunocytochemistry staining and Immunofluorescence detection showed all membrance protein LRRN3 positively expressed cells were sensory neurons in DRG, some weak-positively expressed and some not expressed. Conclusion： The expression of LRRN3 in DRG suggests that LRRN3 is closely associated with embryonic development, morphogenesis and repair following injury of sensory neurons in Dorsal root ganglion.
出处
《现代生物医学进展》
CAS
2011年第23期4405-4408,共4页
Progress in Modern Biomedicine
关键词
LRRN3
人胚
脊神经节
LRRN3
Human embryo
Dorsal root ganglion （DRG）
