摘要
目的 研究纤维连接蛋白受体整合素α５β１ 在大鼠肺纤维化中的作用。方法 用免疫组化方法观察实验性大鼠肺纤维化纤维连接蛋白（ＦＮ） 及其受体整合素α５β１ 和转化生长因子β（ＴＧＦβ）表达的动态变化；用Ｎｏｒｔｈｅｒｎ 印迹杂交和免疫细胞化学方法观察ＴＧＦβ对体外培养的大鼠肺成纤维细胞整合素α５β１ ｍＲＮＡ和蛋白表达的影响。结果 （１）实验组１～３ 天，病灶内上皮细胞和内皮细胞整合素α５β１ 表达明显增强，炎细胞及增生的间质细胞亦呈阳性反应。以后，整合素α５β１ 阳性反应主要见于明显增生的间质细胞，并与ＴＧＦβ阳性分布相似。ＦＮ的变化与整合素α５β１ 的变化同步。（２）ＴＧＦβ作用后，大鼠肺成纤维细胞整合素α５β１ ｍＲＮＡ 及其蛋白表达增强（α５、β１ ｍＲＮＡ 杂交条带的积分光密度值，依次分别是对照的６．６ 、４．４ 倍；α５β１ 蛋白染色积分光密度值为对照的３．６ 倍） 。结论 整合素α５β１在肺间质细胞活化、增殖、分化及细胞外基质合成中起了极其重要作用。
Objective To study the roles of integrin α 5β 1 played in rat pulmonary fibrosis. Methods The dynamic changes of fibronectin (FN), FN receptor α 5β 1, and TGF β were analysed in bleomycin induced rat pulmonary fibrosis by using immunohistochemical technique. Northern blot analysis and immunocytochemistry techniques were performed in order to detect the expression of α 5β 1, FN mRNA and their relevant proteins in cultured rat pulmonary fibrobasts (PFbs) after administration of TGF β1. Results (1) A strong positive reaction with anti integrin α 5β 1 was seen in the pneumocytes, endothelial cells and proliferated mesenchymal cells on the 1st to 3rd day after bleomycin. The positive signals for integrin α 5β 1were apparent mainly in those proliferated interstitial cells with fibroblast and myofibroblast differatiation 7 days after bleomyclin treatment. Changes in FN were similar to that of the integrin α 5β 1. (2) Expression of α 5β 1, FN mRNAs and their relevant proteins was increased in rat PFbs after TGF β1 administration. Conclusions Integin α 5β 1 played a key role in inducing the activation, proliferation and differentiation of PFbs, causing an increase of extra cellular matrix synthesis during pulmonary fibrogenesis.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1999年第6期427-431,共5页
Chinese Journal of Pathology
基金
国家自然科学基金!( 编号39330140)
关键词
肺纤维化
转化生长因子Β
整合素Α5Β1
Pulmonary fibrosis Intercellular adhesion molecule 1 Transforming growth factor beta Immunohistochemistry
