摘要
目的研究美多芭与安坦摄入后帕金森病(parkinson's disease,PD)大鼠行为学及大脑5-羟色胺(5-hydroxytryptamine,5-HT)表达的改变,探讨药物治疗对PD抑郁的影响。方法雄性成年Wistar大鼠30只,随机分为空白对照组与假手术组;PD大鼠45只,随机分为PD组、安坦组和美多芭组,进行行为学、体质量、糖水偏爱测试判断抑郁情况,免疫组织化学检测海马与额叶5-HT的表达。结果 PD组、安坦组和美多芭组平均体质量和糖水偏爱度明显低于空白对照组与假手术组,美多芭组与安坦组糖水偏爱度明显低于PD组(P<0.05),美多芭组大鼠额叶与海马5-HT免疫反应阳性神经细胞数较其他组显著减少(P<0.05)。结论 PD大鼠可表现抑郁,美多芭与安坦治疗加重PD的抑郁症状。美多芭导致额叶与海马5-HT降低,可能与抑郁发生有关。
Objective To study the behavior of rats and hydroxytryptamine（5-HT） expression in their brain,and to analyze the impact of drug therapy on the depression in Parkinson′s disease.Methods Adult male Wistar rats were randomly divided into a blank group and an experimental group,each of 15.For another 90 rats,a Parkinson′s disease model was established for 55,and 45 were taken.For these,3 groups were formed： a PD group,a Madopar group and an Artane group,each of 15.Behavior and sucrose consumption were monitored as means to evaluate the rats′ depression.Expression of 5-HT was examined by immunohistochemistry.Results In body weight,rats in the PD group,Madopar group and Artane group were all lighter than both the blank and experimental groups（P0.05）.In sucrose consumption,the Madopar group and the Artane group were both lower than the PD group,and all these three groups were lower than the blank and experimental groups（P0.05）.Counts for neurons in the hippocampus and frontal lobe,as shown by 5-HT immunohistochemistry,in the Madopar group were lower than all other groups（P0.05）. Conclusion Depression was observed in PD model rats,and Madopar and Artane can aggravate this symptom.Administration of Madopar lowered 5-HT expression in the hippocampus and frontal lobe of the brain,which may contribute to the development of depression.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第11期30-33,共4页
Journal of Shandong University:Health Sciences
基金
山东省优秀中青年科学家科研基金(2006BS03009)
关键词
帕金森病
抑郁
美多芭
安坦
5-羟色胺
Parkinson′s disease
Depression
Madopa
Artane
5-Hydroxytryptamine
