摘要
目的通过不同剂量人工接种,采用人附红细胞体感染移居高原(海拔3 700米)的成年家兔,观察感染后家兔红细胞渗透脆性和血浆游离血红蛋白的变化。方法选取移居高原适应30d以上的成年家兔150只,随机分为5组,每组30只,经耳静脉接种人附红细胞体之后,连续30d观察相关指标,分别于接种前1d、窗口期、高峰期和消失期对家兔耳采静脉抽血测定红细胞渗透脆性及血浆游离血红蛋白。结果窗口期观察2组、观察3组红细胞脆性增加,高峰期各观察组红细胞脆性均增加(P<0.05和P<0.01),消失期各观察组接近接种前和对照组(P>0.05);窗口期、高峰期各观察组血浆中游离血红蛋白均升高,消失期观察2组、观察3组血浆中游离血红蛋白升高(P<0.05和P<0.01),观察1组接近接种前和对照组(P>0.05)。结论高原环境条件下人附红细胞体感染成年家兔,红细胞脆性增加,致红细胞损伤破坏,血浆中游离血红蛋白升高,变化与接种量有关。
Objective To evaluate the influence of human Eperythrozoon on the osmotic fragility of red blood cell（RBC） and the level of plasma free hemoglobin in adult rabbits,immigrated to the plateau with altitude of 3 700 meters.Methods 150 adult rabbits,adapting to plateau migration for more than 30 days,were randomly divided into 5 groups with 30 rabbits for each group and were inoculated via ear vein with human Eperythrozoon.Relevant indicators were observed for 30 days after inoculation,and the osmotic fragility of RBC and the level of plasma free hemoglobin were detected 1 day before inoculation,window period,peak period and disappearance period.Results The osmotic fragility of RBC in observed group 2 and 3 during window period increased,also in all groups during peak period（P0.05 or P0.01）,but that in all observed groups during disappearance period had no significance with that detected before inoculation and that in control group（P0.05）.During window and peak period,the plasma free hemoglobin in all observed groups increased,but during disappearance period,only that in observed group 2 and 3 still remained increasing（P0.05 or P0.01） and that in observed group 1 was close to that detected before inoculation and that in control group（P 0.05）.Conclusion The infection of human Eperythrozoon in adult rabbits at high altitude might increase the osmotic fragility of RBC,causing the destruction of RBC and the increase of plasma free hemoglobin,the changes of which might be related to the inoculation dose.
出处
《国际检验医学杂志》
CAS
2011年第18期2079-2081,共3页
International Journal of Laboratory Medicine
基金
西藏自治区"十五"重点科技项目(2001-100)
关键词
红细胞
兔
创伤和损伤
高原
人附红细胞体
erythrocytes
rabbits
wounds and injuires
plateau
eperythrozoon
