摘要
为进一步提高不同优质肉用品种绵羊的超数排卵与胚胎移植效果,本实验采用4种不同的超排方案(CIDR+FSH减量、CIDR+FSH+PMSG、CIDR+FSH+PG、CIDR+FSH等量)对17只绵羊(杜伯、白萨福克、黑萨福克、德克塞尔、美利奴)进行超数排卵处理,选择体况良好的杂交羊87只做受体,并进行胚胎移植实验。结果表明:第1组(CIDR+FSH减量)和第2组(CIDR+FSH+PMSG)方案超排平均可用胚数分别为6.20、6.60枚,显著高于第3组(CIDR+FSH+PG)和第4组(CIDR+FSH等量)方案(P<0.05);白萨福克、德克塞尔、杜伯超排平均可用胚数分别为7.67、6.20枚和6.25枚,显著高于黑萨福克和美利奴(P<0.05)。供体羊全部发情,得到95枚可用胚胎;51只受体新鲜胚胎移植3个月后经B超检查38只受体怀孕,妊娠率74.5%,产羔率88.2%。因此,CIDR+FSH+PMSG是优质肉绵羊超数排卵方案的最佳组合。
For further improving the quality of superovulation and embryo transfer results of different varieties of donor sheep,four different superovulation programs（CIDR ＋ FSH reduction,CIDR ＋ FSH ＋ PMSG,CIDR ＋ FSH ＋ PG,CIDR ＋ FSH equivalent） were conducted on 17 donor sheep（Du Bo,white Suffolk,Black Suffolk,Texel,Merino）,and 87 good body condition hybrid sheep were choosed as receptors for embryo transfer experiments.The results showed that： the average available embryos number of the first（CIDR ＋ FSH reduction） and the second superovulation group（CIDR ＋ FSH ＋ PMSG）（6.20 and 6.60 pieces） were significantly higher than that of the third（CIDR ＋ FSH ＋ PG） and the fourth superovulation group（CIDR ＋ FSH equivalent）（P 0.05）;the average available embryo number of White Suffolk,Texel,Dubo（7.67,6.20 and 6.25 pieces） were significantly higher than that of black Suffolk and Merino（P0.05）.95 fresh embryos were got from all the donor sheep,and 51 pubescent were transplanted were receptor sheep,which were checked 3 months latter by B-ultrasound,the pregnancy rate was 74.5% and the lambing rate was 88.2%.In conclusion,CIDR ＋ FSH ＋ PMSG is the best combination for superovulation program of high-quality mutton sheep.
出处
《中国畜牧杂志》
CAS
北大核心
2011年第23期25-28,共4页
Chinese Journal of Animal Science
基金
国家科技支撑项目(2008BADB09)
转基因重大专项(2009ZX0800-020B
2008ZX08008-005)
关键词
品种
超数排卵
胚胎移植
绵羊
breed
superovulation
embryo transfer
sheep
