摘要
分析了锁掷酵母中类胡萝卜素的主要代谢途径,研究了碳源、温度、pH、溶氧、有机氮源对锁掷酵母发酵生产类胡萝卜素的影响,发现碳源、温度、pH主要影响菌体的得率;溶氧、有机氮源主要影响类胡萝卜素的组成。可以通过最适的发酵条件单一积累目的类胡萝卜素。以玉米浆为氮源、溶氧控制在5%左右有利于β-胡萝卜素的积累,在优化的条件下,经7L罐补料分批发酵,菌体得率为56.32g/L,β-胡萝卜素产量可达18.92mg/g,占总类胡萝卜素量的60.43%,比优化前提高了217%;当以酵母膏为氮源、溶氧控制在30%左右有利于红酵母烯的积累,在优化的条件下,经7L罐补料分批发酵,菌体得率为62.47g/L,红酵母烯的产量可达31.74mg/g,占总类胡萝卜素的70.41%,比优化前提高了152%。通过优化发酵条件来定向积累单一的类胡萝卜素可为其他菌株生产类胡萝卜素提供一种可行的方法。
Central metabolic pathway of carotenoid was analysed. The production of carotenoids by Sporidiobolus sp. was studied. It was found that biomass was connected with carbon source,temperature and pH,but carotenoids proportion was seriously influenced by dissolved oxygen and nitrogen source. Different carotenoids could be obtained by using selected optimum conditions. In the end,the strategies to produce β-carotene or torulene were obtained. Dissolved oxygen-based fed-batch fermentation in fermentor was used to prove the authenticity of the conclusions. The cell biomass,β-carotene content and β-carotene proportion could reach 56. 32g/L,18. 92mg/L and 60. 43% respectively by using corn steep liquor at 5% of dissolved oxygen saturation. β-carotene content was 271% higher than before. The cell biomass,torulene content and torulene proportion could reach 62. 47g/L,31. 74mg/L and 70. 41% respectively by using yeast extract at 30% of dissolved oxygen saturation. Torulene content was 152% higher than before. The strategy for enhancing specific carotenoid production by selected fermentation conditions might provide an alternative approach to enhance carotenoid production with other strains.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第12期239-242,306,共5页
Science and Technology of Food Industry
