摘要
目的探讨去甲基化药物5-氮杂-2'-脱氧胞苷(5-aza-2'-deoxycytidines,5-Aza-CdR)对食管癌细胞化疗敏感性的影响及其机制。方法经5-Aza-CdR处理人食管癌细胞株EC1后,采用MTT检测各组细胞的化疗敏感性,流式细胞仪分析各组细胞的细胞周期变化,Western blot测定对甲基化转移酶(DNA methyltransferase,DNMT)表达的影响。结果经5-Aza-CdR处理后,EC1细胞对顺铂的化疗敏感性提高。5-Aza-CdR可诱导EC1细胞出现明显的G0/G1期阻滞。DNMT3B在食管癌细胞EC1中表达明显增高,经5-Aza-CdR作用后,其表达水平显著降低。结论 5-Aza-CdR可提高人食管癌EC1细胞对顺铂的敏感性,将细胞阻滞于G0/G1期并下调DNMT3B表达可能是其重要的作用机制。
Objective To investigate the effect of 5-aza-2′-deoxycytidines（5-Aza-CdR） on the chemosensitivity to cisplatin of human esophageal carcinoma EC1 cells in vitro.Methods The chemosensitivity to cisplatin of EC1 cells was examined by MTT assay,cell cycles were detected by flow cytometry and the expression of DNMT（DNA methyltransferase） was measured by Western blot before and after treatment with demethylating agent 5-Aza-CdR.Results After 5-Aza-CdR treatment,the chemosensitivity of EC1 cells to cisplatin was increased and EC1 cells were blocked in G0/G1 phase.The DNMT3B was overexpressed in EC1 cells and was significantly inhibited by 5-Aza-CdR.Conclusion 5-Aza-CdR can enhance the sensitivity of EC1 cells to cisplatin,which may be associated with the induction of cell cycle G0/G1 arrest and the inhibition of DNMT3B expression.
出处
《实用肿瘤杂志》
CAS
北大核心
2011年第6期594-596,共3页
Journal of Practical Oncology
