摘要
目的:建立测定江西不同产地蔓荆子中蔓荆子黄素含量的方法。方法:采用高效液相色谱法。色谱柱为Alltima HP C18(250mm×4.6mm,5μm),流动相为乙腈-50mmol·L-1磷酸二氢钾溶液(磷酸调pH值为3.0)=50∶50,流速为1.0mL·min-1,检测波长为254nm,柱温为室温,进样量为10μL。结果:蔓荆子黄素的进样量在0.2～1.2μg范围内与峰面积积分值呈良好的线性关系(r=0.9994);平均加样回收率为99.96%,RSD=1.42%(n=9)。江西不同产地蔓荆子中蔓荆子黄素含量差异较大。结论:本方法简便、快速、线性关系良好,可用于蔓荆子中蔓荆子黄素的含量测定。
OBJECTIVE： To establish a method for the content determination of vitexicarpin in Vitex trifolia from different pro- ducing areas of Jiangxi province. METHODS： HPLC method was adopted. The determination was performed on Alltima HP C18（250 mm×4.6 mm, 5 μm） column with the mobile phase consisted of acetonitrile-50 mmol.L-1 potassium dihydrogen phosphate solution （pH value adjusted to 3.0 with phosphoric acid, 50：50）, at the flow rate of 1.0 mL.min-1. The detection wavelength was set at 254 urn, and the column temperature was room temperature. The injection volume was 10 μL. RESULTS： The linear range was 0.2～ 1.2μg（r= 0.999 4） with an average recovery of 99.96 % （RSD= 1.42 %, n =9）.Vitexicarpin contents of E trifolia from different areas differed greatly. CONCLUSION： The method is simplp and rapid with good linear relationsgip, which can be used for the content determination of vitexicarpin in V. trifolia.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第47期4489-4490,共2页
China Pharmacy
