摘要
目的 探讨胎儿生长受限以及成年后发生胰岛素抵抗,对雌性大鼠卵巢结构、功能和糖代谢的影响.方法 结扎孕鼠子宫动脉构建胎儿生长受限（FGR）模型.实验对象分为手术组（14只）,对照组（8只）.FGR和对照组新生雌鼠,各取30只,分为两组,给予正常饮食.①检测FGR和对照组大鼠空腹血糖（FPG）及胰岛素水平（FINS）、葡萄糖耐量及葡萄糖钳夹实验,计算稳态葡萄糖输注率（GIR）.评价大鼠胰岛β细胞分泌功能及外周组织胰岛素敏感性;②观测FGR大鼠及对照组大鼠动情周期变化及睾酮（T）水平;③评估两组大鼠卵巢组织形态,采用Westem blot及免疫组化检测卵巢糖原合成蛋白激酶3（GSK-3β）含量和表达.结果 ①FGR大鼠成年后空腹血糖及胰岛素分别为5.60±0.82mmol/L和59.06±14.00mU/L,显著高于对照组的4.55±0.57mmol/L和47.20±15mU/L,t值分别为-3.353和2.203,均P〈0.05）.实验组血糖钳夹实验的GIR（7.95±0.8）mg·kg-1·min-1,低于对照组的（12.99±0.35）mg·kg-1·min-1,P〈0.05;②与对照组相比FGR大鼠动情周期显著延长,睾酮的水平升高,FGR组大鼠动情周期（8.3±1.5）天长于对照组（5.1±0.9天）;③两组大鼠卵巢内均见各级卵泡和黄体存在,两组大鼠卵巢形态学及重量未见明显差异,FGR大鼠卵巢GSK-3β表达显著升高,磷酸化糖原合成蛋白激酶（p-GSK-3β）表达下降.结论 ①FGR雌性大鼠存在成年期胰岛素抵抗;②雌性FGR大鼠可出现动情周期延长,稀发排卵;睾酮、胰岛素水平升高,卵巢局部糖代谢异常等类似多囊卵巢综合征表现.
Objective To explore the influences of fetal growth retardation （FGR） and insulin resistance at their adult stage on ovarian development, function and glucose metabolism in female rats. Methods FGR models were constructed by ligating uterine artery of pregnant rats. The Sprague-Dawley （SD） pregnant rats on day 17 of gestation were divided into experimental group （n = 14） performing bilateral uterine artery- ligation （UAL） and control group （n = 8 ） pedorming sham operation. Thirty newborn female rats were chosen from FGR group and control group respectively, and they were divided into two groups and provided with normal diet. Fasting plasma glucose （FPG） and fasting insulin （FINS） of FGR group and control group were detected, and glucose tolerance test and hyperinsulinemieeuglyeemie clamp test were processed to calculate glucose intake rate （GIR）. Islet β-cell endocrine function and insulin sensitivity in peripheral tissue were evaluated. The change of oestrous cycle and testosterone level were observed in both groups. The ovarian tissues of both groups were evaluated, and Western blot and immunohistochemistry were used to measure the content and expression of GSK-3β. Results FPG and insulin level of adult FGR rats were 5.60 ± 0.82mmol/L and 59.06± 14. 00mU/L respectively, which were significantly higher than those of rats in control group （4.55 ± 0.57mmoL/L and 47.20 ± 15. 00mU/L respectively, t was - 3. 353 and 2. 203, both P 〈 0. 05）. G1R in experimental group was （7.95 ± 0. 80） mg· kg ^-1 min ^- 1, which was lower than that （ 12.99 ± 0.35 ） mg ·kg^- 1 · min ^-1 in control group （ P 〈 0. 05 ）. Compared with that of control group, the estrous cycle of FGR rats prolonged significantly, and testosterone level rose. The estrous cycle of FGR group （8.3 ± 1.5 days） was longer than that of control group （5.1 ±0.9 days）. Ovarian follicle of various stages and eorepus luteum were found in ovary of two groups, but the differences in ovarian morphol
出处
《中国妇幼健康研究》
2011年第6期749-753,共5页
Chinese Journal of Woman and Child Health Research
基金
陕西省科技研究发展（攻关）计划资助项目（2008K14-05）
关键词
胎儿生长受限
胰岛素抵抗
卵巢
生殖
大鼠
fetal growth retardation （FGR）
insulin resistance
ovary
reproduction
rats
