摘要
目的:探讨采用组织块法从根尖牙乳头中分离AP细胞,为牙体及牙髓再生研究奠定基础。方法:前磨牙根尖分离牙乳头;组织块法分离AP细胞,计算集落形成率;免疫荧光、流式细胞仪检测细胞标记物。结果:细胞集落形成率为10～20colonies/103细胞;细胞贴壁生长梭形或多角形核浆比大;原代细胞生长慢,传代后细胞生长快,可传12代以上;细胞表达间质干细胞标记物,AP细胞还表达神经干细胞标记物CD24、Nestin;不表达DSP。结论:组织块法能有效分离牙乳头细胞,AP细胞不同于牙髓干细胞(CD24-)。
Objective： To explore isolation and cultivation of apical dental papilla cells by explant technique.Methods： Apical papilla（AP） was separated from human immature premolar extracted for orthodontic purposes.Explant technique was used to isolate AP cells,then the cells were cultivated in a modification of Eagle＇s medium supplemented with 20%fetal bovine serum.Characterization of the immunophenotype of AP cells was detectded by immunofluorescence and flow cytometry.To evaluate colony-forming efficiency,second passage cells after primary cultivation were used.Results： Colony-forming efficiency of AP cells was 10-20colonies/103 cells plated.AP cells obtained by explant technique were able to adhere to plastic substratum and showed a typical fibroblast-like morphology.AP cells were found to express mesenchymal stem cells markers.In addition,AP cells showed positive staining for Nestin and CD24.Furthermore,cultured AP cells were found not to express dentinogenic marker dentin sialoprotein（DSP）.Conclusion： Explant technique could be used efficiently to isolate AP cells.The phenotype of AP cells is different from DPSCs（CD24-）.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2011年第11期1010-1012,共3页
Journal of Oral Science Research
