摘要
大豆蚜Aphis glycines Matsumura是为害大豆(Glycine max)的重要害虫,已给我国大豆生产造成了较为严重的经济损失。本研究通过测定大豆蚜危害胁迫后大豆叶片可溶性蛋白、非可溶性蛋白、可溶性糖含量及2种防御性酶——过氧化物酶(POD)、过氧化氢酶(CAT)活性的动态变化,探讨大豆蚜危害胁迫对大豆叶片几个重要生理指标的影响。研究结果表明,受到大豆蚜危害胁迫的大豆叶片与未受为害叶片中所含可溶性蛋白含量存在极显著差异(t0.01=11.814,df=4,P<0.01),且前者较后者含量明显增高1.73mg·g-1。与之相比较,非可溶性蛋白与可溶性糖含量分别在此两处理间无显著差异(t0.05=-1.104,df=4,P>0.05;t0.05=-2.639,df=4,P>0.05)。此外,受到大豆蚜危害胁迫的大豆叶片较未受为害叶片中所含POD活性有所升高,但在两处理间POD活性无显著差异(t=-2.639,df=4,P>0.05)。同时,受到大豆蚜危害的大豆叶片较未受为害的大豆叶片中CAT活性增强。本研究有助于揭示大豆蚜对大豆的危害机理,评价不同大豆品种对大豆蚜虫危害的耐受程度,也为大豆抗虫品种选育以及大豆蚜的可持续控制提供理论依据。
The soybean aphid,Aphis glycines Matsumura,is an important agricultural pest in China that causes economically significant damage in some major soybean,Glycine max Merr.（L） ,production areas.In order to determine the effect of A.glycines on the physiology of soybean leaves,we investigated changes in some physio-biochemical indices of leaves,such as soluble and insoluble protein content,soluble carbohydrate（SC） content and the activity of two defensive enzymes;peroxide enzyme（POD） and catalase（CAT） .The results show that heavy infestations of aphids significantly（t0.01=11.814,df=4,P0.01） increased soluble protein levels in soybean leaves compared to aphid-free leaves.However,there was no significant difference in insoluble protein（t0.05=-1.104,df=4,P0.05） or soluble carbohydrate（t0.05=-2.639,df=4,P0.05） between aphid infested and aphid-free leaves.In addition,the results also indicate that levels of POD were higher in aphid-infested leaves than in control leaves,however,this difference was not significant（t0.01=-2.639,df=4,P0.05） .Aphids also caused a marked increase in CAT levels in soybean leaves.These results shed light on the mechanisms by which soybean aphids damage soybeans and also those by which soybeans attempt to protect themselves from aphids.Such information provides a useful basis for selecting insect-resistant soybean cultivars and setting up a theoretical framework for the development of appropriate management strategies.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2011年第6期1655-1660,共6页
Chinese Journal of Applied Entomology
基金
公益性行业(农业)科研专项(201103022)
关键词
大豆蚜
危害胁迫
生理指标
防御性酶
Aphis glycines
herbivore stress
physiological indexes
defensive enzyme
