摘要
目的探讨重组人5型腺病毒对A549细胞增殖、细胞周期及凋亡的影响。方法采用MTT法、细胞迁徙试验、流式细胞术、TUNEL法等方法检测重组人5型腺病毒对A549细胞生物学特性的影响。结果重组人5型腺病毒在体外对A549细胞有显著的生长抑制作用,且具有时间和浓度依赖性;一定质量浓度的重组人5型腺病能显著降低A549细胞迁徙活性,诱导细胞凋亡,阻滞A549细胞于G0/S期。结论重组人5型腺病在肿瘤的临床治疗中有着重要的作用和广阔的前景。
Objective To investigate the influence of recombinat human adenovirus type 5 on cell proliferation,cell cycle and apoptosis of A549 cells in vitro.Methods We examined the effect of recombinat human adenovirus type 5 on the cell biological characteristics of A549 cells in vitro with the methods of MTT,cell migration assay,flow cytometry and TUNEL assay.Results Recombinat human adenovirus type 5 had a significant inhibition effect with time-and concentration-dependence for A549 cells in vitro,at the same time,recombinat human adenovirus type 5 at a certain concentration could inhibit cell migration process significantly,and induced G0/S phase arrest and cell apoptosis under the same conditions.Conclusion Recombinat human adenovirus type 5 has an important role in the clinical therapy of tumor and wide prospect.
出处
《中国药业》
CAS
2011年第22期32-33,共2页
China Pharmaceuticals
