摘要
以羽叶薰衣草（Lavandula pinnata L.）茎段为外植体进行离体培养,探讨羽叶薰衣草外植体的消毒灭菌、增殖培养、生根培养、炼苗移栽等一系列技术措施。结果表明：用0.1%HgCl2处理羽叶薰衣草外植体6~8 min的效果较好;外植体在含0.8 mg/L 6-BA及0.1 mg/LNAA的MS培养基中培养,增殖效果较好,培养42 d后,平均增殖系数达11.3;较合适的试管苗生根培养基为含0.2 mg/L NAA的1/2 MS培养基,培养21 d后,生根率为98.2%,平均每株生根12.5条;炼苗后移栽成活率达95.0%以上。
The in vitro culture of Lavandula pinnata L.was established by using the nodal stem segments.The method of sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting,the hardening and transplantation of test-tube plantlets were investigated.The results showed that soaking the explants in 0.1% HgCl2 for 6~8 minutes was a reliable method for obtaining sterile explants.Initial sterile culture was performed on MS medium supplemented with 0.8 mg/L 6-BA and 0.1 mg/L NAA for 42 days,which average a multiplication coefficient of 11.3.For root formation,the optimum medium was 1/2 strength MS supplemented with 0.2 mg/L NAA,which resulted in the rooting rate of 98.2% within 21 days of culture,and each plantlet generated 12.5 roots on average.After hardening,transplanted plantlets could achieve a higher survival rate at 95.0%.
出处
《现代农业科技》
2011年第22期50-51,共2页
Modern Agricultural Science and Technology
基金
苏州大学第十三批大学生课外学术科研基金
关键词
羽叶薰衣草
组织培养
炼苗
移栽
Lavandula pinnata L.
tissue culture
hardening
transplantation
