摘要
本研究旨在探讨在具有不同超数排卵效果的绵羊品种中,在进行超排处理后其卵巢上的促黄体素受体(LH receptor,LHR)分布情况,为进一步研究二者的相关性以及据此为改进超排效果奠定基础。根据超数排卵效果的优劣依次选择3个我国地方绵羊品种:小尾寒羊、白滩羊和乌珠穆沁羊。在常规超排最后一次注射FSH后的8、16和24h分别各取3只绵羊的卵巢,采用免疫组织化学法观察3个品种绵羊卵巢上LHR的分布情况。结果表明:LHR存在于3个地方品种绵羊卵巢有腔卵泡的颗粒细胞和膜细胞上,但在早期卵泡上不表达;各品种绵羊的近成熟卵泡上LHR阳性细胞率都显著高于相应的次级卵泡(P<0.05);3品种绵羊近成熟卵泡中LHR的阳性变化率存在显著差异,从高到低依次为小尾寒羊>白滩羊>乌珠穆沁羊(P<0.05)。籍此可以得出以下结论:LH对绵羊早期卵泡发育的诱导可能不需要通过其特异性受体起作用;3品种绵羊近成熟卵泡中LHR的阳性变化率与超排效果是一致的,表明超数排卵的效果可能与绵羊卵巢上的LH受体分布存在一定的联系。
In this study,it was aimed to investigate the distribution of luteinizing hormone receptor（LHR） on the ovaries among three different sheep breeds by different efficiency of superovulation.Three local sheep breeds（Small-tailed Han sheep,White Tan sheep and Ujumqin sheep） were selected depending on their different sensitivity to superovulation by LH as following： Small-tailed Han sheep,White Tan sheep,and Ujumqin sheep.At 8,16,24 hour after the last injection with LH,the sheep ovaries were collected and treated by immunohistochemical staining to observe the distribution of LHR on their different ovarian follicles.The results showed that： ① LHR were expressed in both granulosa and theca cells of antral follicles,but did not express on the pre-antral ones.②At 8,16,24 hour after the last injection with LH,the LHR levels on the pre-matured follicles of the three local sheep breeds were significantly higher than those on the corresponding secondary ones respectively（P0.05）.③The expression change of LHR in the pre-matured follicles had significant difference among the three sheep breeds,the order as following： Small-tailed Han sheepWhite Tan sheepUjumqin sheep（P0.05）.The result indicate that： ①The induction of LH for the development of the primary ovarian follicles may not be through the specific LH receptor signal way,but through some other non-specific ones.②The expression change of LHR in the pre-matured follicles is consitent with the effect of superovulation,which indicate that the effect of superovulation is linked with the distribution of LH receptor on ovaryes in sheep.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2011年第11期1625-1631,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"863计划"重点项目(2008AA101003)
2009年度国家自然基金面上项目(30871836)
2009年度北京市自然基金重点项目(5091002)
关键词
绵羊
超数排卵
促黄体素受体
免疫组织化学染色
sheep
superovulation
luteinizing hormone receptor
immunohistochemical staining
