摘要
测定和比较了远海梭子蟹(Portunus pelagicus)成熟卵巢和早期胚胎的水分、总脂、脂类组成和脂肪酸含量,结果表明:早期胚胎的水分含量显著大于成熟卵巢,但后者总脂/组织湿重含量显著小于前者,两者的总脂/组织干重并无显著差异;甘油三酰和磷脂含量在早期胚胎与成熟卵巢之间并不存在显著差异,但是早期胚胎中的游离脂肪酸和胆固醇含量均显著高于成熟卵巢,这可能是胚胎发育过程中脂类物质被水解成游离脂肪酸、胆固醇和甘油等物质;就脂肪酸组成而言,两者中均检测出27种已知脂肪酸,仅15∶0、14∶1n7、20∶1n7和18∶3n3 4种脂肪酸的含量存在显著差异(P<0.05),16∶0、18:0、16∶1n7、18∶1n9、20∶5n3和22∶6n3为成熟卵巢和早期胚胎中的主要的脂肪酸种类,其中n3-PUFA/n6-PUFA和20∶5n3/22∶6n3分别为3.5和0.7。
The present experiments were conducted to investigate the moisture content,lipid class and fatty acid composition in mature ovary and early embryo of Portunus pelagicus.The results indicated that：（1） Although the early embryo of P.pelagicus had higher moisture content than that of mature ovary,the higher total lipid/wet tissue could be found in the mature ovary than that of early embryo;（2） There were no significatn differences in the triacyglycerol（TG） and phospholipids（PL） contents between mature ovary and early embryo,however,the early embryo had higher free fatty acids and cholesterol contents than the mature ovary.This could be explained by the hydrolyzation of TG and PL;（3） There were twenty seven identified fatty acids that could be detected in the both mature ovary and early embryo.The significant differences existed only in 15∶ 0,14∶ 1n7,20∶ 1n7 and 18∶ 3n3 between mature ovary and early embryo while the main fatty acids were 16∶ 0,18∶ 0,16∶ 1n7,18∶ 1n9,20∶ 5n3 and 22∶ 6n3 for both tissues.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2011年第6期853-857,共5页
Journal of Shanghai Ocean University
基金
上海市晨光计划(2007CG64)
国家教育委员会博士点基金(200802640002)
上海市高校创新团队项目(D-8006-10-209)
关键词
远海梭子蟹
成熟卵巢
早期胚胎
脂肪酸
脂类组成
Portunus pelagicus
mature ovary
early embryo
fatty acid
lipid class composition
