摘要
应用Ames试验和小鼠活体骨髓多染红细胞微核试验,研究了生育调节剂环氧司坦的诱变作用。Ames试验选用TA_(97a)、TA_(98)、TA_(100)、TA_(102)菌株,剂量浓度5、50、250、500、1000μg/JШ。微核试验剂量为240、480、960mg/kg·w。结果,加和不加 S_9Ames试验的回复突变菌数均无明显增加。微核试验三个剂量组的平均微核率分别为0.21±0.14、0.24±0.15、0.28±0.15、与溶剂对照0.41±0.11比较,经统计学分析P值均大于 0.05(p>0.05),无显著性差异。研究表明,环氧司坦对原核细胞和真核细胞无诱变作用。
The mutagenic activity of epostane agent was studied by measuring the reverse gene mutation in Salmonella thphimurium and micronuclei of bone marrow in vivo in mice.
The TA97a, TA98, TA1000, TA102 of bacterial strains were used in Ames test both with and without metabolic activation. A total number of 48 animals were randomly divided into five groups of control, positive and 3 doses of epostane agent. The positive mutagens were used as control as follows. TA97a 9-amino-acridine 50μg/plate, TA98 P-nitroquinoline 200μg/plate, TA100 methl-methansufo-nat lug/plate, TA102 mitomycin C 5μg/plate in wothout S9 and TA97a, TA98, TA100 2-aminofluorene 10μg/plate, TA102 1.8-dihydroxyanthroquiuone 50μg/plate in with S9. Micronucleus test was also used mitomxcin C 4mg/kg.w. as positive control.
The result showed that the mutagens-induced mutagentsis were increased in the all bacterial strains, but had no marked increased levels of epostane agent at 5, 2550, 0, 500, 1000μg/plate. Similarly, the high frequencyes of micrnouclear appeared in mitomycin C and they were only 0.21±0.14, 0.24 ± 0.15, 0.28±0.15 at 240, 480, 960mg/kg.w of epostane agent, there were no significantly difference between them(P>0.05)in comparison with 0.14 ± 0.11 of the control respectively.
This paper indicated that the epostane of the fertility requ-lation agent did not possess mutagenic activity both for procaryotes and eucaryotic cell.
出处
《癌变．畸变．突变》
CAS
CSCD
1990年第4期36-38,91-92,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
