摘要
用0.58mol/L NaCl沉淀提取Tamm-Horsfall糖蛋白(THP)制备了三株抗THP单克隆抗体(McAb)。免疫印迹技术(Immunoblotting Technigue,IBT)证明这些McAb特异性地和94kD THP抗原结合,ELISA抑制试验表明三株McAb识别THP抗原的不同位点。用McAb双夹心ELISA法测定尿THP的含量,表明尿THP含量与血尿素氮(BUN)、血肌酐(Scr)呈显著负相关(P<0.01),与内生肌酐清除率(Ccr)呈明显正相关(P<0.01),BUN、Scr、Ccr正常的各种肾病尿THP含量无明显异常(P<0.05)。测定尿THP含量可作为判断肾功能损害程度的良好指标。
Urinary Tamm-Horsfall protein (THP) was prepared by precipition with 0.58 NaCl. Splenocytes from BALB/C mice immunized with THP were fused with myeloma cell Sp2/0 and were cultured in HAT.Three hybridomas capable of producing monoclonal antibodies (McAb) (H-5, A4-8 and D-8) were obtained Western-blotting showed that these McAbs reacted specifically with 94kd THP. Competition-ELISA demonstrated that these McAbs recognized different epitopes. The sandwich ELISA was applied for detection of urinary THP with three anti-THP McAbs. The clinical investigation showed that urinary THP contents correlated negatively with blood urea nitrogen (BUN) and serum creatinine (Scr) levels (p<0.01) and correlated positively with creatinine clearance (Ccr) (p<0.01). When BUN, Scr and Ccr were normal in renal patients, the urinary THP contents were not statistically different from those in the control group. This study suggests that variation of urinary THP is an significant data in detecting renal function damage.
出处
《现代免疫学》
CAS
CSCD
北大核心
1991年第4期208-211,共4页
Current Immunology
