摘要
通过对西蒙得木〔Simmondsia chinensis(Link)〕组织培养大批量繁殖技术的研究,探索基本培养基、生长调节物质及蔗糖含量等对嫩梢增殖及生根的影响,结果表明:采用春季生长的1年生实生苗以及6年生高产的优株茎节作外植体,培养于含有ZT 1～3 mg/L或BA 1～3mg/L和NAA 0.01～0.1 mg/L的MS培养基中,经30～40天,几乎所有腋芽均能长成嫩梢,切取长达4～6 cm的嫩梢,培养于含有NAA的1/2MS培养基中,经1周暗培养,1个月后生根率达93％,小植株移栽于含砂土的基质中,保持湿润,获得成功。用幼苗及优株作外植体的培养效果无显著差异,说明此法不受遗传型的限制。
This paper reports a method for mass micropropagation of jojoba. Plant growth regulators, basal media and sugar level were examined to determine their effects on shoot growth, shoot multiplication and rooting in vitro. The results showed that the nodel explants from 1 year old seedlings grown in spring and 6 years old, high yield, individual trees were cultured on MS medium supplemented with ZT 1~3mg/L or BA 1~3 mg/L and NAA 0.01~0.1 mg/L. Shoots were obtained from all the axillary buds after 30~40 days of culture. Shoots measuring about 4~6cm can be excised and rooted in 1/2 MS medium added with NAA following one week in dark for rooting. About 93 % rooting was achieved after one month. The plantlets were placed into moist chamber with sand-soil medium successfully. No major differences on in vitro propagation between young seedlings and adult plants of jojoba were found. It appears that the method may not be limited by genotype.
出处
《林业科学研究》
CSCD
北大核心
1991年第5期512-516,590,共6页
Forest Research
关键词
西蒙得木
组织培养
繁殖
外植体
jojoba (Simondsia chinensis)
tissue culture
propagation
exptants
