摘要
本文应用ABC-ELISA检测乙型肝炎e抗原、e抗体,结果表明,抗-HBc的最佳生物素标记用量为150μg/mg Ab,最适包被、标记抗体应用浓度均为10μg/ml。该法和普通ELISA比较,在294份血清中。抗-HBe的阳性率分别为59.18％及34.35％;209份HRsAg阳性血清中,HBeAg阳性率各为34.93％和27.75％,P<0.01,ABC法具有较高的灵敏度;在20份ABC法HBeAg阳性而普通法阴性的血清中,用抗-HBe作中和抑制试验,抑制率均>50％;本法检测抗-HBe时批内CV为9.23％,批问为9.80％,检测HBeAg时批内CV7.42％,批间为6.43％。
We used ABC-ELISA for the detection of hepatitis Be antigen and antibody. The results showed that the best labelling quantity of BNHS of HBeAb was 150 μg/mg antibody. The optimum concentration of both the coating and labelling antibodies were 10 μg/ml. We compared ABC-ELISA with conventional ELISA and found that of 294 serum samples, HBeAb positive rates were 59.18% and 34.35% respectively, and of 209 HBsAg positive samples HBeAg positive rates were 34.93% and 27.75% (P<0.01), so ABC-ELISA was more sensitive. In 20 samples which were shown HBeAg positive by ABC method but negtive by the other, the results of neutralization tests by HBeAb were all positive. When ABC method was used for detecting HBeAb, the CV in the same groups was 9.23%, and that between groups was 9.80%. When it was used for detecting HBeAg, the CV was 7.42% and 6.43% respectively.
出处
《海军医学杂志》
1991年第4期337-340,共4页
Journal of Navy Medicine
