摘要
采用市售抗HCVELISA试剂盒,葡萄球菌A蛋白(SPA)菌体及酶标记抗人IgM建立血清抗HCV·IgM 检测 SPAELISA。以多种实验对其方法的特异性进行了考核。将其用于临床检测,急肝患者抗 HCV·IgM 检出率明显高于抗 HCV·IgG(11/16,8/16,P<0.05),输血相关性肝炎、散发性肝炎及其高频度社交人群两者的检出率分别为 42.86％与 78.57％,27.27％与 36.36％,及 0与8.47％。且急肝患者抗 HCV·IgM 阳性率显著地高于慢性肝炎患者。
A new assay for detection of specific TgM antibody against HCV (anti-HCV IgM) in serum has been developed using the second generation anti-HCV ELISA kit (specific for anti-HCV IgG) .Straptococus protein A bearing germs and peroxidase labelled goat anti human IgM(HRP-GAHM). The specificity of this new method was proved by multiple sub stitution tests,block tests,and a distroying test with β-mecaptoethanol (2-ME). The detection rates of anti-HCV IgM and anti-HCV IgG were 68. 75% and 50% in acute hepatitis pa tients ,42. 86% and 78. 57% in transfusion related hepatitisones,27. 27% and 36. 36% in sporadic hepatitis ones,and zero and 8. 47% in the population with active social activities, respectivley. On the other hand, the positive rate of anti HCV IgM in acute hepatitis (68. 75%) was higher than in chronic hepatitis (27. 08%).
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
1993年第S1期61-64,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
肝炎
病毒性
人
肝炎抗体
免疫球蛋白类
μ链
酶连接免疫吸附测定
hepatitis, viral, haman
hepatitis antibodies
immunoglobulins, Mu chain
enzyme-linked immunosorbent
