摘要
对牙周炎病牙根面作单纯手器刮治(A),加用构橼酸(B)。加用构橼酸与Fn(C)处理后,用细胞培养方法观察3种处理方法对成纤维细胞的移行、附着、生长的影响。通过^(51)铬标记成纤维细胞,测定细胞的放射活性,计算细胞附着率。结果显示:1天时A、B、C组分别为49.77％、52.65％、55.69％,4天时为38.09％、47.25％、45.0％,6天时为23.08％、70.96％、58.23％。B、C组3个时相均高于A组,4、6天时与A组有显著性差异(P<0.01)。实验证明,牙周炎病牙根面的生物学处理可使部分牙骨质脱矿和有机基质暴露,改变了生物学活性,对成纤维细胞的移动、附着、增殖有重要意义。
This study evaluated the effects os fibreblasts migration and growth on Root faceof periodontal disease in vitro. The sick root slices were treated alone sealing and root planing and partial demineralixation with citric acid and fibronection and were divided into A, B, G groups. The fibroblasts were explanted on the upper root slice surface and cultured. After 1, 4, 6 days the fi- broblasts were observed and labelled 51 Cr and rate of cell attachment by gammacunts on root slice surface. The results show rate of cell attachment on the root slice were more significant in B and C groups than in A, and group C is higher triple than group A on the sixth day. Experimental evidence suggests that the partial demineralization of cementum and fibronection treatment were exposed dentinal collagenous and promoted cell of attachment.
出处
《口腔材料器械杂志》
1993年第2期10-11,13+47-48,共5页
Chinese Journal of Dental Materials and Devices
关键词
成纤维细胞
根面处理
部分脱矿
fibroblast, root surface treatment, partial demineralization.
