摘要
应用图象分析技术对正常口腔粘膜、口腔扁平苔藓及其伴有不同程度上皮异常增生和恶变的病理切片行Feulgen染色后,测量细胞核的形态参数(包括核面积、周长、最大径、最小径、等效圆直径、形状因子)和DNA含量。以5CER作为区分良恶性病变的指标,5CER≥10％为恶性,5CER<10％为良性。从本文分析中光镜检查结果与图象分析结果有差异。由于用光镜定性分析人为因素较多;因此,探索一条新的诊断途径是非常必要。作者应用定量与定性分析同步进行,可以纠正定性分析中的主观因素,对指导临床治疗、判断预后有很重要的意义。
After the Feulgen dying, if the normal oral mucous, oral Lichen planus as well as the pathological cut sheets with atypical dysplasia and malignant change in different degrees, use the image analysis technique to measure the morphologic parameters of the nucleus (including nuclear surface area, perimeten, maximum nuclear diameter, minimum nuclear diameter, equivalent circle diameter, morphological factor) and the amount of DNA. 5CER acts as an index of distinguishing malignancy or benignancy, the value of 5CER in malignant is more than 10% and in benignant is less than 10%. The result of the analysis from the optical microscope differs from the image analysis. There are so many personal factors in the qualitative analysis by optical microscope that it because quite imperative to research for a new diagnosing way. The synchronization of quantitative analysis and qualitative analysis can correct the personal errors in qualitative analysis. It will be very useful for directing the clinical practice and the judgement of prognosis.
出处
《上海口腔医学》
CAS
CSCD
1993年第1期32-34,60-61,共5页
Shanghai Journal of Stomatology
