摘要
采用 PCR 技术体外 DNA 扩增,检测结核分枝杆菌,扩增片断为165 bp,非肺结核分枝杆菌及其它细菌结果阴性,证实其具有较高的特异性。通过与常规 DNA 制备方法的比较,证明对脑脊液标本煮沸后进行直接圹增,方便、快捷,节省费用,不影响实验结果,值得推广。通过对33例结核性脑膜炎(结脑)及26例非结脑患者脑脊液进行结核分枝杆菌 DNA 检测,并与其它诊断方法进行比较,结果显示 PCR 的阳性率为84.8%(28/33),染色镜检3%(1/33),抗原检测60.6%(20/33),抗体检测54.5%(18/33),对照组无一例扩增阳性,表明 PCR 技术在特异性和敏感性方面均优于其它方法(P<0.01),可望成为结脑可靠的诊断手段。
A polymerase chain reaction (PCR) for the specific detection of Mycobacterium tuberculosis has been developed and evaluated for the clinical applicability from among 9 different DNA templates test (including 5 species of Myeobacteria).PCR amplified the DNA from M.tuberculosis only, demonstrating its exquisite specificity.We have developed a simple,rapid,and reliable method for isolation of DNA.A total of 59 clinical specimens were analysed.It consisted of 33 tuberculous meningitis specimens (test group) and 26 non-tuberculous meningitis specimens(control group).The PCR in cerebrospinal fluid was compared with conventional bacteriology,Dot-immunobinding technique(DIBT) for antigen and a ABC-ELISA for antibodies in the diagnosis of tuberculous meningitis.PCR was the most sensitive technique;it detected 28(84.8%)of 33 cases of TBM.DIBT detected 20(60.6%) of all 33 Cases,ABC-ELISA detected 18(54.5%)in all,acid-fast staining detected 1 (3%)of in the same samples (P<0.01).None of the specimens in the control group was positive by PCR.We concluded that PCR is an extremely suitable tool for the diagnosis of TBM.
出处
《医学研究生学报》
CAS
1993年第1期26-28,共3页
Journal of Medical Postgraduates
关键词
脑膜炎
结核性
多聚酶链式反应
脑脊液
tuberculosis
meningitis
polymerase chain reaction
cerebrospinal fluid
