摘要
应用反转录－聚合酶链式反应（ＲｅｖｅｒｓｅＴｒａｎｓｃｒｉｐｔｉｏｎ一ＰｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＲＴ一ＰＣＲ）方法，亚克隆出大鼠小脑和肾脏ＮＯＳｃＤＮＡ，并以此为探针，通过Ｎｏｒｔｈｅｒｎｂｌｏｔｔｉｎｇ分析和定量ＰＣＲ方法，测定大鼠中枢和外周一些组织中ＮＯＳｍＲＮＡ的含量，证明ＮＯＳｍＲＮＡ广泛存在于中枢和外周组织中，在中枢以小脑含量为最高，在外周组织中以肾脏含量为最高；定量ＰＣＲ方法测定ＮＯＳｍＲＮＡ较Ｎｏｒｔｈｅｒｎｂｌｏｔｔｉｎｇ分析更为灵敏。
With Reverse
Transcription- Polymerase Chain Reaction(RT- PCR),nitric oxide
synthase(NOS) com-plementary DNA has been subcloned from rat
cerebellum and kidney.The levels of NOSmRNA in some cen-trical and
peripheral tissues in rat have been detectd by Northern blotting and
quantitative PCR.It has been proved that NOS mRNA is existed
extensively in centrical and peripheral tissues.Among of them,NOS mR-
NA Ievels are highest in cerebellum in centre and in kidney in
peripheral tissues.Moreover, quantitative PCR is more sensitive than
Northern blotting in detecting NOS gene expression.
出处
《北京大学学报（医学版）》
CAS
CSCD
1994年第S1期1-4,共4页
Journal of Peking University:Health Sciences
关键词
一氧化氮合成酶
MRNA
定量PCR
Nitric oxide synthase
mRNA
Quantitative polymerase chain
reaction.
